Alkaline Phosphatase
ROCHE/10567752001 - EIA grade, from calf intestine
Synonym: AP; Alkaline Phosphatase
Product Type: Chemical
| biological source | bovine (calf) intestine |
| form | solution |
| manufacturer/tradename | Roche |
| optimum pH | 9.8 |
| packaging | pkg of 1.5 mL (15 mg) |
| pH range | 9.0-11.0 |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer) |
| Application: | Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis. |
| General description: | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) Amino groups: 8-16 moles/mole enzyme |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, ready-to-use, 10mg/ml |
| Preparation Note: | Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C. 1:10 dilution in water will give stability for 1 week at 4°C. Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity. |
| Quality: | Purity: single peak (HPLC) |
| Sequence: | Purification procedure AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol. In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP No information in literature about -SH groups in AP. Carbohydrate structure Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available. NH2- and carbohydrate estimation Results of NH2- and carbohydrate estimation are available. |
| Storage and Stability: | Store at 2 to 8 °C. (Do not freeze) |
| Unit Definition: | Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5] 1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions] |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer) |
| Enzyme Commission (EC) Number | 3.1.3.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |