Chromozym PK
ROCHE/10378445001 - 90% (enzymatic), powder, pkg of 20 mg
Synonym: C33H39N8O6COOCH3
Product Type: Chemical
| assay | 90% (enzymatic) |
| description | Molecular Formula: C33H39N8O6COOCH3 |
| form | powder |
| manufacturer/tradename | Roche |
| mol wt | Mr 702.9 |
| packaging | pkg of 20 mg |
| Quality Level | 100  |
| storage temp. | room temp |
| Application: | Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma. |
| General description: | Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein. |
| Legal Information: | Chromozym is a registered trademark of Pentapharm AG, Basel |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+) Working concentration: Approximately 0.5 mM Storage conditions (working solution): 2 to 8 °C An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C. |
| Principle: | Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml. |
| Quality: | Contaminant: <0.5% free 4-nitraniline |
| Sequence: | Benzoyl-prolyl-phenalanyl |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 90% (enzymatic) |
| Storage Temp. | room temp |
| UNSPSC | 12352204 |