5-Bromo-2′-deoxyuridine
ROCHE/10280879001 - >97%, crystalline solid, pkg of 1 g
Synonym: 5-Bromo-2′-deoxyuridine; brdu; 5-BrdU; 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil; 5-Bromouracil deoxyriboside; BUdR
CAS Number: 59-14-3
Empirical Formula (Hill Notation): C9H11BrN2O5
Molecular Weight: 307.10
MDL Number: MFCD00006529
Linear Formula: C9H11BrN2O5
Product Type: Chemical
| assay | >97% |
| form | crystalline solid |
| InChI | 1S/C9H11BrN2O5/c10-4-2-12 |
| InChI key | WOVKYSAHUYNSMH-RRKCRQDMSA |
| manufacturer/tradename | Roche |
| mol wt | 307.1 |
| mp | 191-194 °C (dec.) (lit.) |
| packaging | pkg of 1 g |
| Quality Level | 100  |
| shipped in | wet ice |
| SMILES string | OC[C@H]1O[C@H](C[C@@H]1O) |
| storage temp. | 2-8°C |
| Application: | 5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies. |
| Application: | Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method. |
| Biochem/physiol Actions: | Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase. |
| General description: | It is conventionally used as a marker for S-phase synthesis of DNA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used. According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation. Preparation of Working Solution Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration. Storage conditions (working solution): -15 to -25 °C |
| Quality: | Purity: 97% (from N), chromatographically homogeneous |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H351 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% |
| mp | 191-194 °C (dec.) (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |