Pyrophosphatase, inorganic (PPase)
ROCHE/10108987001 - from yeast
Synonym: inorganic pyrophosphatase
Product Type: Chemical
| biological source | yeast |
| form | suspension |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mL (1 mg) |
| Quality Level | 100  |
| specific activity | ~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.) |
| storage temp. | 2-8°C |
| Application: | Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies. |
| Application: | Pyrophosphatase, inorganic (PPase) has been used in : • Enzymatic nitric oxide (NO) detection system • In vitro transcription • Multiply-primed rolling circle amplification |
| Biochem/physiol Actions: | Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. |
| Biochem/physiol Actions: | Mg ions are necessary for catalytic activity of crystalline pyrophosphatase. |
| General description: | Pyrophosphate phosphohydrolase |
| General description: | The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Quality: | Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate). |
| Specificity: | Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.6.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |