cOmplete™ Protease Inhibitor Cocktail
ROCHE/04693116001 - Tablets provided in EASYpacks
Synonym: protease inhibitor
Product Type: Chemical
cOmplete
Inhibition of different proteases by cOmplete Protease Inhibitor Tablets One cOmplete tablet was added per 50 mL incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25 °C.
| absorption | 0.08 at 280 nm |
| form | tablet |
| manufacturer/tradename | Roche |
| packaging | pkg of 20 tablets |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| Application: | cOmplete™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts. cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps. cOmplete contains EDTA (1 tablet yields a 1 mM EDTA solution in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography. Alternatively, the product cOmplete, EDTA-free, can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is present. cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet, is identical to the normal cOmplete Tablet. |
| Application: | cOmplete™ Protease Inhibitor Cocktail has been used as an component in • extraction buffer for protein extraction from Arabidopsis seedling • in radioimmunoprecipitation assay (RIPA) buffer for the isolation of protein from lymphocytes • the extraction buffer for the lysis of protoplast. |
| Components: | Proprietary mixture of several protease inhibitors with broad inhibitory specificity. |
| Features and Benefits: | Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results. • Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer. • Complete protection: Instantly protect your proteins against a broad range of proteases. • Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. • Safe: Choose non-toxic inhibitors that pose no risk to you or those around you. |
| General description: | cOmplete Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete tablets contain EDTA and both irreversible and reversible protease inhibitors. Protease inhibitors present in cOmplete Tablets do not form irreversible complexes with the SH groups of proteins. |
| Legal Information: | cOmplete is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. |
| Physical form: | Tablets in foil blister packs |
| Preparation Note: | Working concentration: 1 tablet per 50ml extraction solution Working solution: Handling Carefully push the tablet through the foil packaging using the base of your thumb (not finger nail) to prevent the breakage of tablets. Preparation of Working Solution One cOmplete tablet is sufficient for the inhibition of the proteolytic activity in 50ml extraction solution. When a very high proteolytic activity is present, one tablet should be used for 25ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared. Preparation of Stock solution (25 X conc.) Dissolve one cOmplete tablet in 2ml dist. water or in 2ml 100 mM phosphate buffer, pH 7.0. Storage conditions (working solution): The stock solution can be stored at 2 to 8°C for 1 to 2 weeks, or at least 12 weeks at -15 to -25°C. |
| Reconstitution: | Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C. |
| Specificity: | Inhibits a broad spectrum of serine proteases, cysteine proteases, and metalloproteases, as well as calpains. |
| Storage and Stability: | Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible) |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H314 |
| Precautionary statements | P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |