DIG Oligonucleotide Tailing Kit, 2nd generation
ROCHE/03353583910 - sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| Quality Level | 100 |
| storage condition | avoid repeated freeze/thaw cycles |
| usage | sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide) |
| Application: | DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in: • northern blot assay • in situ hybridization (ISH) • fluorescence in situ hybridization (FISH) In addition to common hybridization techniques, DIG-labeled oligonucleotides are especially useful for screening expression libraries for sequence-specific DNA binding proteins, for example, transcription factors. |
| Features and Benefits: | Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100). • Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides • Fast hybridization kinetics, due to the small size of oligonucleotides • Single-stranded probes, no renaturation during hybridization • Sequence can be designed according to the experiment • Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells |
| General description: | DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 11 components |
| Preparation Note: | Working concentration: Oligonucleotides In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied. |
| Principle: | DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s). |
| Storage and Stability: | Store at -15–-25 °C. (unopened kit) |
| Symbol |    GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 + H332 - H350i - H360F - H411 |
| Precautionary statements | P201 - P261 - P273 - P280 - P308 + P313 - P391 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 12352200 |
| Components | Reaction Buffer 5x concentrated; CoCl<SUB>2</SUB> Solution 25 mM; DIG-dUTP Solution 1 mM; dATP Solution 10 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl; Control DNA 0.25 mg/ml; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA; Poly(A) Solution 10 mg/ml |