Azure A chloride
ALDRICH/861049 - certified by the BSC, Dye content 70 %
Synonym: Methylene blue
CAS Number: 531-53-3
Empirical Formula (Hill Notation): C14H14ClN3S
Molecular Weight: 291.80
EC Number: 208-510-7
MDL Number: MFCD00012112
Linear Formula: C14H14ClN3S
Product Type: Chemical
| ε (extinction coefficient) | ≥11000 at 242-246 nm in H2O at -1 |
| ≥26000 at 287-291 nm in H2O | |
| ≥34000 at 620-634 μm in H2O | |
| λmax | 633 nm |
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | blue to very dark blue |
| very dark green | |
| composition | Dye content, 70% |
| form | powder |
| InChI | 1S/C14H13N3S.ClH/c1-17(2) |
| InChI key | NALREUIWICQLPS-UHFFFAOYSA |
| mp | 290 °C (dec.) (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | NC1=CC(SC(C2=N3)=CC(C=C2) |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| Application: | Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles. |
| Application: | Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears. |
| Biochem/physiol Actions: | The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye. |
| Packaging: | 1, 5, 25 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 290 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 52005 |
| UNSPSC | 12171500 |

