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Sulforhodamine B

ALDRICH/230162 - Dye content 75 %

Synonym: Acid Red 52; Sulforhodamine B monosodium salt

CAS Number: 3520-42-1
Empirical Formula (Hill Notation): C27H29N2NaO7S2
Molecular Weight: 580.65
EC Number: 222-529-8
MDL Number: MFCD00010180
Linear Formula: C27H29N2NaO7S2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-230162-1G 1 g
 $40.20
1/EA		 Add To Favorites
45-230162-5G 5 g
 $124.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 230162-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 230162-5G

 

ε (extinction coefficient) ≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
λmax 554 nm
application(s) diagnostic assay manufacturing
hematology
histology
assay ≥73% (HPLC)
color brown to very dark brown
  green to very dark green
composition Dye content, 75%
form powder
InChI 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
InChI key SXQCTESRRZBPHJ-UHFFFAOYSA-M
Quality Level 100 
SMILES string [Na+].CCN(CC)c1ccc2c(OC3=CC(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](CC)CC)c1
solubility methanol: 1 mg/mL
storage temp. room temp
technique(s) microbe id | staining: suitable
Application: Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.
General description: Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.
Packaging: 1, 5 g in glass bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥73% (HPLC)
Storage Temp. room temp
Colour Index Number 45100
UNSPSC 12171500

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