Isolation of total RNA from soil supernatants
- Rapid and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
- Easily lyse difficult gram positive cells, plants, and other cellular material
- Lysis and purification solutions protect RNA during processing
- Humic acids levels enormously reduced to allow uninhibited RT-PCR
- From lysis to total RNA in less than 2 hours
The FastRNA® Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil sample supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by the FastPrep Instrument in impact-resistant 2 ml tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX® and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.
Kit Components: RNApro™ Solution, Lysing Matrix E, Inhibitor Removal Solution, DEPC-treated H2O, RNAMATRIX® Binding Solution, RNAMATRIX Slurry, RNAMATRIX Wash Solution Concentrate, Quick-Clean Spin Filters
Total RNA extracted from Soil Samples with the FastRNA Pro Soil-Indirect Kit. Approximately 15% of the total RNA isolated from four different soil samples was loaded on to a 0.8% agarose gel. Lane 1: 1kb ladder, Lane 2: RNA from 5 g Soil #1, Lane 3: RNA from 10 g Soil #2, Lane 4: RNA from 5 g Soil #7, Lane 5: RNA from 5 g Soil #10.
RT-PCR of Fungal Gene from Total RNA Isolated from Soil Samples with the FastRNA Pro Soil-Indirect Kit. Approximately 40% of the RT-PCR reaction was loaded on to a 0.8% agarose gel. Lane 1: 150bp - 2kb marker, Lane 2: Soil #1, Lane 3: Soil #2, Lane 4: Soil #7, Lane 5: Soil #10.