Isolation of total RNA from yeast and fungi
- Rapid and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
- Spores and other difficult cells lysed with ease
- RNA safely and consistently isolated with single-reagent RNApro™ Solution
- Lyses, isolates and precipitates RNA in less than 90 minutes
The FastRNA® Pro Red Kit is designed to efficiently isolate total RNA from yeast and fungi. Using the FastPrep Instrument, up to 109 cells are homogenized by Lysing Matrix C in impact-resistant 2 ml tubes. Total RNA is released into the proprietary, protective RNApro Solution. Following extraction with chloroform and precipitation, total RNA is suitable for RT-PCR analysis and other downstream applications.
Yeast and fungal total RNA extracted with the FastRNA Pro Red Kit. Approximately 2% of the total RNA isolated from 100 mg tissue or 1010 cells was loaded onto a 1.2% denaturing agarose gel (1X MOPS). Lane 1: S. pombe; Lane 2: S. cerevisiae; Lane 3: P. pastoris; Lane 4: C. albicans; Lane 5: common mushroom; Lane 6: small common mushroom; Lane 7: 0.24-9.5kb RNA Ladder.
Kit Components: Lysing Matrix C, RNApro™ Solution, DEPC-treated H2O