Isolation of total RNA from plant and animal samples
- Rapid and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
- Difficult samples such as roots, stems, seeds, and cartilage lysed with ease
- RNA safely and consistently isolated with single-reagent RNApro™ Solution
- Lyses, isolates and precipitates RNA in less than 90 minutes
The FastRNA® Pro Green Kit is designed to efficiently isolate total RNA from any type of plant and animal tissue or cultured cells. Using the FastPrep Instrument, between 50-500 mg of tissue can be homogenized by Lysing Matrix D in impact-resistant 2 ml tubes. Total RNA is released into the proprietary, protective RNApro Solution. Following extraction with chloroform and precipitation, total RNA is suitable for RT-PCR analysis and other downstream applications.
Rat total RNA extracted with the FastRNAPro Green Kit. Approximately 2% of the total RNA isolated from 100 mg frozen tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). Lane 1: tail; Lane 2: kidney; Lane 3: liver; Lane 4: ear; Lane 5: brain; Lane 6: 0.24-9.5kb RNA Ladder.
Plant total RNA extracted with the FastRNA Pro Green Kit. Approximately 2% of the total RNA isolated from 100 mg tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). Lane 1: wheat seed; Lane 2: tomato leaf; Lane 3: red soybean seed; Lane 4: oat seed; Lane 5: iceplant leaf; Lane 6: barley seed; Lane 7: 0.24-9.5kb RNA Ladder.
Kit Components: Lysing Matrix D, RNApro™ Solution, DEPC-treated H2O