Isolation of total RNA from gram positive and gram negative bacteria
- Rapid and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
- Difficult gram positive cells lysed with ease
- RNA safely and consistently isolated with single-reagent RNApro™ Solution
- Lyses, isolates and precipitates RNA in less than 90 minutes
The FastRNA® Pro Blue Kit is designed to efficiently isolate total RNA from gram positive and gram negative bacteria. Using the FastPrep Instrument, up to 1010 cells are homogenized by Lysing Matrix B in impact-resistant 2 ml tubes. Total RNA is released into the proprietary, protective RNApro Solution. Following extraction with chloroform and precipitation, total RNA is suitable for RT-PCR analysis and other downstream applications.
Bacterial total RNA extracted with the FastRNA Pro Blue Kit. Approximately 2% of the total RNA isolated from 1010 bacterial cells was loaded on to a 1.2% denaturing agarose gel (1X MOPS). Lane 1: Salmonella typhimurium; Lane 2: Pseudomonas stuartii; Lane 3: Escherichia coli; Lane 4: Bacillus subtilis; Lane 5: 0.24-9.5kb RNA Ladder.
Kit Components: Lysing Matrix B, RNApro™ Solution, DEPC-treated H2O