Isolation of DNA from cells present in soil or other environmental samples
- Rapid and reproducible sample lysis with the FastPrep® Instrument
- Easily isolates DNA from a variety of organisms in many different types of soil
- Lyses and isolates DNA in less than 60 minutes
- No hazardous organic reagents required
The FastDNA® SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples. Up to 500 mg soil can be processed by the FastPrep® Instrument in impact-resistant 2 ml tubes. The released DNA is purified by a GENECLEAN® procedure and is suitable for PCR analysis and other downstream applications.
DNA from various soil samples extracted with the FastDNA® SPIN Kit for Soil. 20% of the DNA isolated from 500 mg soil was loaded on a 1.2% agarose gel (0.5X TAE). Soil was taken from: Lane 1: tomato pot; Lane 2: sludge; Lane 3: sandy soil; Lane 4: under pine tree; Lane 5: under palm tree; Lane 6: green garden; Lane 7: Nile Lilly pot; Lane 8: lawn grass; Lane 9: citrus tree; Lane 10: avocado tree. DNA ranges from approximately 4-20 kb.
Kit Components: Lysing Matrix E, PPS Solution, Binding Matrix, SEWS-M Solution, DES Solution, Sodium Phosphate Buffer, BBS Solution, MT Buffer, SPIN Filters, Catch Tubes