Isolation of genomic DNA from plants, animals, bacteria, yeast, algae, and fungi
- Rapid and reproducible sample lysis with the FastPrep® Instrument
- Lyses and isolates DNA in less than 60 minutes from a variety of sample types
- Flexible format eliminates need for separate DNA isolation kits
- No hazardous organic reagents required
The FastDNA® Kit is used with the FastPrep® Instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 60 minutes. Four lysing matrix combinations can be generated, and the kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types. The released DNA is purified by a silica-based GENECLEAN® procedure and is suitable for PCR analysis and other downstream applications.
Kit Components: Lysing Matrix A, Binding Matrix, SEWS-M Solution, DES Solution, BBS Solution, CLS-VF Solution, PPS Solution, CLS-TC Solution, CLS-Y Solution
DNA from plant samples extracted with the FastDNA® Kit. Approximately 1 µg of isolated DNA was loaded on a 1.2% agarose gel (0.5X TAE). Lane 1: ˜0.16g apple stem; Lane 2: ˜0.45g red bell pepper seeds; Lane 3: ˜0.45g pelargonium root; Lane 4: ˜0.45g mature peace lily leaf; Lane 5: ˜0.45g ice plant leaf; Lane 6: Lambda Hind III marker.
DNA from animal samples extracted with the FastDNA® Kit. Approximately 1 µg of isolated DNA was loaded on a 1.2% agarose gel (0.5X TAE). Lane 1: ˜0.4g rat liver; Lane 2: ˜0.5g mouse brain; Lane 3: ˜0.45g chicken bone; Lane 4: Lambda Hind III marker.