Anti-Glucagon antibody, Mouse monoclonal
SIGMA/SAB4200685 - clone K79bB10, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Glucagon antibody produced in mouse; GCG; GLP1; GLP2; GRPP
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat pancrease sections with 10 μg/mL Monoclonal Anti-Glucagon, Clone: K79bB10 (Cat. No. SAB4200685). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | K79bB10, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | cat, rabbit, mouse, guinea pig, rat, dog, porcine, zebrafish, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 5-10 μg/mL using heat−retrieved formalin-fixed, paraffin-embedded Rat pancreas sections and Biotin/ExtrAvidin®-Peroxidase staining system. |
| radioimmunoassay: suitable |
| Application: | Monoclonal Anti-Glucagon antibody produced in mouse has been used in immunofluorescence staining. The product may be used in several immunochemical techniques including immunohistochemistry and radioimmunoassay (RIA). |
| Biochem/physiol Actions: | The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis and ketogenesis. Glucagon has an antagonistic effect in the maintenance of normoglycemia to those of insulin and is suppressed by GLP1. Thus up-regulation of glucagon is leading to increased blood glucose levels. Glucagon-specific antibodies would prove useful as a Langerhans islets α cell, glucagonomas and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glucagon is a hormone peptide produced in Langerhans islets α-cells in the pancreas. The active form of glucagon is derived from the cleavage of preproglucagon (GCG). Additional cleavage peptides are oxyntomodulin and glucagon-like peptide-1 (GLP1). |
| Immunogen: | Polymerized porcine glucagon |
| Legal Information: | ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |