Anti-Biotin antibody, Mouse monoclonal
SIGMA/SAB4200680 - clone BN-34, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Biotin antibody produced in mouse; Bios II; Coenzyme R; D-Biotin; Vitamin B7; Vitamin H
Product Type: Chemical
ELISA Monoclonal Anti-Biotin Clone: BN-34 (Cat. No. SAB4200680) was serially diluted to detect Biotin by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BN-34, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human, bovine, rat, mouse |
| storage temp. | −20°C |
| technique(s) | ELISA: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: 0.15-0.3 μg/mL | |
| western blot: suitable |
| Biochem/physiol Actions: | Biotin functions as a coenzyme for acetyl CoA, pyruvate, propionyl-CoA and 3-methylcrotonyl-CoA carboxylases for the regulation of gluconeogenesis, lipid homeostasis, pleotropic gene expression and to replenish the intermediates of tricarboxylic cycle. Biotin bound to histones plays an important role in chromatin remodeling, DNA repair and repression of transposons to maintain genome stability. Inadequate biotin levels may lead to dermatitis, conjunctivitis, alopecia, and central nervous system abnormalities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Biotin is a bi-heterocyclic, essential water soluble vitamin and a cofactor synthesized in most of the plants and prokaryotes. Protein-bound biotin in usually found in dietary sources like meat, cereals, egg yolk, liver and some vegetables. |
| Immunogen: | biotinylated KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |