Anti-CT-Pro ET1 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200665
Synonym: C-terminal pro-endothelin 1
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Placenta sections with 20 μg/mL Rabbit Anti-CT-Pro ET1 (C-terminal) (Cat. No. SAB4200665). The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence A549 cells were fixed and permeabilized using cold methanol followed by acetone. Fixed cells were stained with 10 μg/mL Rabbit Anti-CT-Pro ET1 (C-terminal) (Cat. No. SAB4200665). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Recombinant human Endothelin peptide (amino acid 168-212) was separated on SDS-PAGE and probed with Rabbit Anti-CT-Pro ET1 (C-Terminal) (Cat. No. SAB4200665). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/m antibody 3. 0.25 μg/mL antibody 4. 0.5 μg/mL antibody+ CT-Pro ET1 (C-Terminal) immuinzing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| mol wt | antigen ~5 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoblotting: 0.5-1 μg/mL using the C-terminal region of human recombinant CT-Pro ET1 peptide |
| immunofluorescence: 5-10 μg/mL using A549 cells | |
| immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed and paraffin-embedded human placenta. | |
| UniProt accession no. | P05305  |
| Application: | Anti-CT-Pro ET1 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including immunoblotting using the C-terminal region of human recombinant CTPro ET1 peptide (~5 kDa), immunohistochemistry and immunofluorescence. |
| Biochem/physiol Actions: | Endothelin 1 (ET1) is a vasoconstrictor and is involved in pulmonary vascular homeostasis. ET1 has been linked to HIV (human immunodeficiency virus)-associated pulmonary arterial hypertension and endothelial dysfunction. |
| Biochem/physiol Actions: | Endothelin-1 (ET-1) plays an important role in various tissues. It is involved in pathogenesis of cardiovascular diseases. Indeed, circulating ET-1 levels are increased in most cardiovascular diseases. The active ET-1 peptide and C-terminal-pro-ET1 (CT-pro-ET1) peptide plasma levels are elevated in heart failure and after acute myocardial infarction (AMI), and give prognostic information on mortality. CT-proET-1 may represent a clinically useful marker of prognosis after AMI. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Endothelin 1 (ET1) is an endothelium-derived peptide. It is synthesized as an inactive form of 212 amino acids and later converted to an active 21 amino acid peptide. The gene encoding it is localized on human chromosome 6p24.1. |
| General description: | Endothelin-1 (ET-1), also known as Preproendothelin-1 (PPET1). PPET1 undergoes several proteolytic cleavages to yield ET-1. Endothelin-1 belongs to the Endothelin family of potent vasoconstrictor peptides. It consists of three structurally-related members, distinct in their pharmacological activities and distribution ET-1, ET-2 and ET-3. |
| Immunogen: | a synthetic peptide corresponding to the C-terminal region of CT-Pro ET1, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |