Anti-AOX1 antibody, Mouse monoclonal
SIGMA/SAB4200562 - clone AO15, purified from hybridoma cell culture
Synonym: Monoclonal Anti-AO; Monoclonal Anti-AOH1; Monoclonal Anti-AOX1 antibody produced in mouse; Monoclonal Anti-EC 1.2.3.1; Monoclonal Anti-aldehyde oxidase 1
Product Type: Chemical
Immunoblotting Whole extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-AOX1 Clone: AO15 (Cat. No. SAB4200562). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + 90 μg/mL AOX1 immunizing peptide (human, 566-580) 3. 1 μg/mL antibody + 90 μg/mL AOX1 immunizing peptide (human, 1316-1330)
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AO15, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~148 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1.0-2.0 μg/mL using extracts of HepG2 cells. |
| UniProt accession no. | Q06278  |
| Application: | Monoclonal Anti-AOX1 antibody produced in mouse has been used in reverse-phase protein array analysis(5) and immunoblotting. |
| Biochem/physiol Actions: | Aldehyde oxidase 1 (AOX1), along with the endoplasmic cytochrome P450 system (CYP450), participates in the drug metabolism and generation of reactive oxygen species. It also has a role in the hepatic phase I metabolism of numerous xenobiotics. Reduced AOX1 protein expression is implicated in chronic pancreatitis, and hepatocellular carcinoma. Furthermore, hypermethylation of the AOX1 promoter region is observed in colon cancer and prostate cancer, making it a potential biomarker for prostate cancer diagnosis. Polymorphism in the AOX1 gene also displays variation in metabolism of the quinoxaline phenoxypropionic acid derivative XK469. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-AOX1 (mouse IgG1 isotype) is derived from the hybridoma AO15 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Aldehyde oxidase 1 (AOX1) comprises the 2Fe/2S clusters and two flavin adenine nucleotide (FAD) domain-containing N-terminal domain I and II, respectively. The C-terminal domain encompasses domain III with a substrate-binding site. The gene encoding AOX1 is mapped to human chromosome 2q33.1. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human AOX1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-AOX1 recognizes human AOX1. |
| Storage and Stability: | For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,isnot recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |