Anti-ARG1 antibody, Mouse monoclonal
SIGMA/SAB4200508 - clone ARG1-3, purified from hybridoma cell culture
Synonym: Anti-arginase-1; Anti-liver-type arginase; Anti-type I arginase; Monoclonal Anti-ARG1 antibody produced in mouse
Product Type: Chemical
Immunoblotting Whole extract of rat liver was separated on SDS-PAGE and probed with Monoclonal Anti-ARG1 Clone:ARG1-3 (Cat. No. SAB4200508). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + ARG1 immunizing peptide
Immunoprecipitation Monoclonal Anti-ARG1 Clone: ARG1-3 (Cat. No. SAB4200508) was used to immunoprecipitate ARG1 from HEK-293T cells overexpressing human ARG1 whole cell extract. Detection antibody: Rabbit Anti-ARG1 (Cat. No. SAB4200510). Lanes 1. Positive control: whole cell extract 2. 5 μg antibody 3. 10 μg antibody 4. Negative control: 5 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ARG1-3, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 2-4 μg/mL using whole extracts of rat or mouse liver. |
| UniProt accession no. | P05089  |
| Application: | Monoclonal Anti-ARG1 antibody produced in mouse has been used in immunoblotting. |
| Biochem/physiol Actions: | Arginase hydrolyzes arginine to ornithine and urea. Arginases have been implicated in many disease processes, including vascular diseases, pulmonary diseases, infectious diseases, immune cell dysfunction and cancer. Inherited deficiency of ARG1 enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ARG1 (mouse IgG1 isotype) is derived from the hybridoma ARG1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Arginase (ARG)1 gene, codes for type I isoform of arginase. ARG1 is a cytosolic enzyme expressed predominantly in the liver as a component of the urea cycle. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal region of human ARG1, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse ARG1 and by 4 amino acids in rat ARG1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |