Anti-ATG101 antibody, Mouse monoclonal
SIGMA/SAB4200506 - clone ATG101-15, purified from hybridoma cell culture
Synonym: Anti-C12orf44, chromosome 12 open reading frame 44, PP894, Atg13-interacting protein; Anti-
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human ATG101 was separated on SDS-PAGE and probed with Monoclonal Anti-ATG101 Clone: ATG101-15 (Cat. No. SAB4200506). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative control: without 1st antibody
Immunoprecipitation Monoclonal Anti-ATG101 Clone: ATG101-15 (Cat. No. SAB4200506) was used to immunoprecipitate ATG101 from HEK-293T cells overexpressing human ATG101 whole cell extract. Detection antibody: Rabbit Anti-ATG101 (Cat. No. SAB4200175). Lanes 1. Positive control: whole cell extract 2. 10 μg antibody 3. 5 μg antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ATG101-15, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~30 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells overexpressing human ATG101. |
| Application: | Monoclonal Anti-ATG101 antibody produced in mouse is suitable for use in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Atg101 is a mammalian autophagy-related 13 (Atg13) binding protein essential for autophagy. It is associated with the Unc-51 like autophagy activating kinase 1 (ULK1), Atg13, focal adhesion kinase (FAK) family kinase-interacting protein of 200 kDa (FIP200) complex via direct interaction with Atg13. Autophagy is an evolutionarily conserved pathway seen in all eukaryotic cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ATG101 (mouse IgG1 isotype) is derived from the hybridoma ATG101-15 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Atg101 is conserved in humans and several eukaryotes. It is not conserved in yeast Saccharomyces cerevisiae. ATG101 gene is located on the human chromosome at 12q13.13. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human ATG101, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, canine and bovine ATG101. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti- ATG101 recognizes human ATG101. |
| Storage and Stability: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |