Anti-ADAR1 antibody, Mouse monoclonal
SIGMA/SAB4200482 - clone ADAR61, purified from hybridoma cell culture
Synonym: Anti-ADAR1; Anti-DRADA; Anti-DSH; Anti-DSRAD; Anti-G1P1; Anti-IFI4; Anti-K88DSRBP; Anti-P136; Monoclonal Anti-ADAR1 antibody produced in mouse
Product Type: Chemical
Immunoblotting Whole extract of U-2-OS cells was separated on SDS-PAGE and probed with Mouse Anti-ADAR1 Clone: ADAR61 (Cat. No. SAB4200482). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 4 μg/mL antibody + 90 μg/mL ADAR1 immunizing peptide (human, 483-499) 3. 4 μg/mL antibody + 90 μg/mL ADAR1 immunizing peptide (human, 1075-1094)
Immunoblotting Lysates were separated on SDS-PAGE and probed with 4 μg/mL Mouse Anti-ADAR1 Clone: ADAR61 (Cat. No. SAB4200482). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HepG-2 2. U-2-OS 3. 3T3 4. Mouse total brain lysate 5. Rat total brain lysate 6. Rat total liver lysate 7. COS-7 8. MDBK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ADAR61, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3 |
| mol wt | antigen 80-130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human, rat |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 2.0-4.0 μg/mL using U2OS total cell extracts | |
| UniProt accession no. | P55265  |
| Application: | Monoclonal Anti-ADAR1 antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • flow cytometry |
| Biochem/physiol Actions: | RNA editing by hydrolytic deamination of adenosine (A) to inosine (I) in double-stranded (ds)RNA is the most common type of editing in higher eukaryotes. This RNA editing event is catalyzed by the adenosine deaminase that acts in RNA (ADAR) enzyme. Two ADAR enzymes have been shown to possess enzymatic activity in mammals, ADAR1 and ADAR2. Both can convert specific adenosine to inosine in pre-mRNA and can also convert up to 40-50% of the adenosines in long synthetic duplex RNAs. ADAR1 plays critical roles in differentiating cells of embryo and adult tissues, supporting cell survival and permitting their further differentiation and maturation. Its role in embryonic development is especially demonstrated within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 is also an essential regulator of hematopoietic stem cell maintenance and suppressor of interferon signaling that may protect organisms from the deleterious effects of interferon activation associated with many pathological processes, including chronic inflammation, autoimmune disorders and cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ADAR1 (mouse IgG3 isotype) is derived from the hybridoma ADAR61 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Adenosine deaminase that acts in RNA 1 (ADAR1) and ADAR2 are expressed in most tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include ADAR1 (150kDa) and two short isoforms (80 and 110 kDa) that are functional. |
| Immunogen: | a peptide corresponding to a sequence at the C-terminus of human ADAR, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |