Anti-ADFP / PLIN2 antibody, Mouse monoclonal
SIGMA/SAB4200452 - clone ADFP-5, purified from hybridoma cell culture
Synonym: Adipophilin; Plin2 Antibody; Plin2 Antibody - Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse; Anti-ADRP; Anti-Perilipin 2; Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse
Product Type: Chemical
Immunofluorescence SHSY-5Y cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Monoclonal Anti-ADFP/PLIN2 Clone: ADFP-5 (Cat. No. SAB4200452). The antibody was developed using Goat Anti-Mouse IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HEK-293T cells overexpressing human ADFP/PLIN2 was separated on SDS-PAGE and probed with Monoclonal Anti-ADFP/PLIN2 Clone: ADFP-5 (Cat. No. SAB4200452). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + ADFP/PLIN2 immunizing peptide
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-ADFP/PLIN2 Clone: ADFP-5 (Cat. No. SAB4200452). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T cells overexpressing human ADFP/PLIN2 2. SHSY-5Y
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ADFP-5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~48 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HepG2 or SH-SY5Y cells |
| western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human ADFP | |
| UniProt accession no. | Q99541  |
| Application: | Monoclonal Anti-ADFP / PLIN2 antibody produced in mouse may be used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Perilipin 2 (PLIN2) plays a vital role in uptake and storage of lipids as triacylglycerols in lipid droplets in skeletal muscle. PLIN2 is also involved in the regulation of lipid metabolism. High levels of PLIN2 is beneficial for the prevention of insulin resistance. Overexpression of PLIN2 is associated with clear cell renal cell carcinoma (CC-RCC). |
| Biochem/physiol Actions: | PLIN2 may serve as a biomarker of lipid accumulation in diverse cell types and diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-ADFP/PLIN2 (mouse IgG1 isotype) is derived from the hybridoma ADFP-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. |
| General description: | Perilipin 2 (PLIN2) is a 50 kDa major protein belonging to perilipin family. It is present on lipid droplets found in the cytoplasm of variety of cells as hepatocytes, adipocytes, muscle cells, mammary epithelial cells, fibroblasts, endothelial cells and macrophages. PLIN2 gene, spanning 14 kb, with eight exons, is located on human chromosome 9p22.1. |
| Immunogen: | a synthetic peptide corresponding to a sequence at the C-terminus of human PLIN2, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-ADFP/PLIN2 recognizes human ADFP/PLIN2. |
| Storage and Stability: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |