ProteinA-Sepharose® 4, Fast Flow from Staphylococcus aureus
SIGMA/P9424 - aqueous ethanol suspension
Synonym: Protein A–Agarose
MDL Number: MFCD00165563
Product Type: Chemical
| analyte chemical class(es) | proteins (Immunoglobulins of various mammalian species) |
| biological source | Staphylococcus aureus |
| capacity | ≥30 mg/mL binding capacity (human IgG) |
| extent of labeling | ~6 mg per mL |
| form | aqueous ethanol suspension |
| matrix | Sepharose 4B Fast Flow |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| matrix spacer | 1 atom |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children. |
| Application: | Protein A-Sepharose® Fast Flow from Staphylococcus aureus has been used: • in co-immunoprecipitation assay • in immunodepletion • to purify IgG from human serum and plasma |
| General description: | Protein A-Sepharose® has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children. |
| Legal Information: | Sepharose is a registered trademark of Cytiva |
| Packaging: | 1 mL in glass insert |
| Packaging: | 5 mL in glass bottle |
| Physical form: | Suspension in 20% ethanol |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P243 |
| Risk Statements | 10 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 104.0 °F |
| Flash Point(C) | 40 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |