Protein G Sepharose®, Fast Flow
SIGMA/P3296 - recombinant, expressed in E. coli, aqueous ethanol suspension
Synonym: Protein G-Agarose, Fast Flow from Streptococcus sp.
MDL Number: MFCD00165580
Product Type: Chemical
| analyte chemical class(es) | proteins (Immunoglobulins of various mammalian species) |
| extent of labeling | ~2 mg per mL |
| form | aqueous ethanol suspension |
| matrix | Sepharose 4B Fast Flow |
| matrix activation | cyanogen bromide |
| matrix attachment | amino |
| matrix spacer | 1 atom |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| Application: | Protein G-Sepharose® is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose® has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum. |
| General description: | Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG. |
| General description: | Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG. P3296-5Ml′s updated product number is GE17-0618-01 |
| Legal Information: | Sepharose is a registered trademark of Cytiva |
| Packaging: | 1 mL in glass insert |
| Packaging: | 5 mL in glass bottle |
| Physical form: | Suspension in 20% ethanol |
| Preparation Note: | Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Risk Statements | 10 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 115.0 °F - closed cup |
| Flash Point(C) | 46.1 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |