Anti-Pro-BDNF antibody produced in rabbit
SIGMA/P1374 - affinity isolated antibody, lyophilized powder
Synonym: Anti-
MDL Number: MFCD08705507
Product Type: Chemical
Immunohistochemistry Anti-proBDNF: Cat. No. P1374: Immunohistochemical staining of Rat cerebellum with Anti-proBDNF antibody. proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.
Immunoblotting Anti-proBDNF: Cat. No. P1374: Western blot analysis of Rat glioma C6 (lanes 1,3) or human neuroblastoma SH-SY5Y (lanes 2,4) cell lysate. Lanes 1,2. Anti-proBDNF antibody, (1:200). 3,4. Anti-proBDNF antibody, preincubated with the control peptide antigen.
Immunoblotting Anti-proBDNF: Cat. No. P1374: Western blot analysis using Anti-proBDNF antibody, (1:400). Lanes 1. proBDNF (WT-human), (20 ng). 2. proNGF (WT-human), (200 ng). 3. recombinant proNT-3 (200 ng). 4. hBDNF, (200 ng). 5. mNGF 2.5S (Grade II), (200 ng). 6. hNT-3, (200 ng).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:200 using rat glioma C6 and human neuroblastoma SH-SY5Y cell lines |
| western blot: 1:400 using recombinant mouse proBDNF | |
| UniProt accession no. | P23560  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | Brain derived neurotrophic factor (BDNF) belongs to a family of secreted proteins called neurotrophins. Neurotrophins are synthesized as preproneurotrophin precursors and further processed to proneurotrophins and subsequently to neurotrophins. BDNF is expressed in the developing and the mature brain. The influence of BDNF is mediated by its binding to TrkB receptor and sometimes the receptor p75NTR. The downstream pathways promoted by BDNF include PI3K, MAPK and JAK/STAT. The most important role of BDNF is the regulation of synaptic transmission and plasticity, protein synthesis and phosphorylation of local proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Does not cross react with pro-NGF, pro-NT-3, or mature BDNF. |
| General description: | The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Does not cross react with pro-NGF, pro-NT-3, or mature BDNF. |
| Immunogen: | peptide corresponding to amino acid residues 72-88 of human BDNF (precursor). This sequence has 16/17 residues identical in mouse and rat. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, with 1% bovine serum albumin and ≤0.1% sodium azide. |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 22-36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |