Lipopolysaccharides from Salmonella enterica serotype typhimurium
SIGMA/L6511 - purified by phenol extraction
Synonym: LPS
MDL Number: MFCD00164404
Product Type: Chemical
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: L6511-100MG
| biological source | Salmonella enterica (Serotype typhimurium) |
| color | white to faint yellow |
| form | lyophilized powder |
| impurities | <3% Protein (Lowry) |
| purified by | phenol extraction |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | water: 4.90-5.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow |
| storage temp. | 2-8°C |
| Application: | Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). |
| Biochem/physiol Actions: | Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics. |
| General description: | This product is phenol extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS has been used to induce NOS in rats and guinea pigs. |
| Other Notes: | To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page. |
| Packaging: | 10, 25 mg in poly bottle |
| Packaging: | 100 mg in glass bottle |
| Preparation Note: | The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 + H312 + H332 |
| Precautionary statements | P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |