Anti-HSPA13 antibody produced in rabbit
SIGMA/HPA014797 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Microsomal stress 70 protein ATPase core; Anti-STCH antibody produced in rabbit; Anti-Stress 70 protein chaperone microsome-associated 60 kDa protein precursor
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neuronal cells.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts and leydig cell.
Immunohistochemistry Immunohistochemical staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | AYGLHKADVFHVLVIDLGGGTLDVS |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:20-1:50 |
| UniProt accession no. | P48723  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | HSPA13 (heat shock protein 70kDa family, member 13) is responsible for processing both secretory and cytoplasmic proteins. It binds to misfolded or denatured peptides, and then uses ATP-derived energy to release them. Unlike other members of HSP70 family, which are induced by heat shock, this protein is activated by Ca2+ ionophore A23187. Studies in Japanese population show that variants of this gene might be linked to susceptibility to gastric cancer. This protein is capable of modulating TRAIL-mediated cell apoptosis, and hence, plays a key role in cell survival. It interacts with pH-regulating transporters NBCe1-B and NHE1, and modulates their membrane expression. Therefore, it regulates intracellular pH during stress, and facilitates cellular recovery from acidification. Mutations in this gene have been shown to be associated with Alzheimer′s disease, epilepsy and autism. It is also thought to be involved in regulating plasticity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | HSPA13 (heat shock protein 70kDa family, member 13) belongs to the heat shock protein 70 chaperone family. It has a highly conserved N-terminal domain, which contains the ATPase domain, and a unique hydrophobic leader sequence. It is a constitutively and ubiquitously expressed gene, and the encoded protein has a molecular weight of 60kDa. This gene is localized to human chromosome 21q11.1. It is localized to the microsomal region of the cell. |
| Immunogen: | Stress 70 protein chaperone microsome-associated 60 kDa protein precursor (Microsomal stress 70 protein ATPase core). |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72076 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |