Anti-CDK7 antibody produced in rabbit
SIGMA/HPA007932 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-39 kDa protein kinase antibody produced in rabbit; Anti-CAK antibody produced in rabbit; Anti-CAK1 antibody produced in rabbit; Anti-CDK-activating kinase antibody produced in rabbit; Anti-Cell division protein kinase 7 antibody produced in rabbit; Anti-P39 Mo15 antibody produced in rabbit; Anti-STK1 antibody produced in rabbit; Anti-TFIIH basal transcription factor complex kinase subunit antibody produced in rabbit
MDL Number: MFCD00282167
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and liver tissues using HPA007932 antibody. Corresponding CDK7 RNA-seq data are presented for the same tissues.
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell lines Caco-2 and MCF-7 using Anti-CDK7 antibody. Corresponding CDK7 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry Immunohistochemical staining of human liver shows very weak positivity in hepatocytes as expected.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GCILAELLLRVPFLPGDSDLDQLTR |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | P50613  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | CDK7 (cyclin-dependent kinase 7) gene encodes a serine/threonine kinase that is a member of the cyclin-dependent protein kinase (CDK) family. CDKs bind to cyclin, get activated and mediate cell cycle progression. CDK7 interacts with Cyclin H and MAT1 to form a trimeric complex that functions as cyclin-dependent kinase (CDK) activating kinase (CAK) that activates phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulates cell cycle. CAK is essential for the progression of cell cycle. CDK7 is also a component of the transcription factor TFIIH that plays a role in initiation of transcription and DNA repair. CDK7 may serve as a target for cancer therapy. It may also function in transcriptional repression by inhibiting RNA polymerase II-catalyzed transcription. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Cell division protein kinase 7 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST86954 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |