Anti-AKAP12 antibody produced in rabbit
SIGMA/HPA006344 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-A-kinase anchor protein 12 antibody produced in rabbit; Anti-A-kinase anchor protein 250 kDa antibody produced in rabbit; Anti-AKAP 250 antibody produced in rabbit; Anti-Myasthenia gravis autoantigen gravin antibody produced in rabbit
MDL Number: MFCD08705585
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA006344 antibody. Corresponding AKAP12 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human endometrium, kidney, skeletal muscle and testis using Anti-AKAP12 antibody HPA006344 (A) shows similar protein distribution across tissues to independent antibody HPA056230 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell line U-251 MG and human cell line MCF-7.
Immunohistochemistry Immunohistochemical staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human endometrium shows moderate positivity in cells in endometrial stroma.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong positivity in cells in glomeruli.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | QRAEAERPEEQAEASGLKKETDVVL |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | Q02952  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | AKAP12 (A-kinase anchoring protein 12) interacts with the RII subunit of protein kinase A (PKA), and also interacts with PKC, phosphodiesterase 4D, Ca2+/calmodulin, and the β2-adrenergic receptor. Thus, it has multiple essential roles in cellular processes. It sequesters Src protein to caveolin-rich lipid rafts, and thus, prevents the interaction of Src with FAK-associated adhesion and signaling complexes. Therefore, it inhibits the movement of tumor cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | AKAP12 (A-kinase anchoring protein 12) is a scaffold protein, and is also called gravin in humans. It has a wide range of tissue expression. Its subcellular distribution depends upon its isoform, which are three in number- α, β and γ. It has a molecular weight of 300kDa. |
| Immunogen: | A-kinase anchor protein 12 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST86532 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |