Anti-ACSL4 antibody produced in rabbit
SIGMA/HPA005552 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-LACS 4 antibody produced in rabbit; Anti-Long-chain acyl-CoA synthetase 4 antibody produced in rabbit; Anti-
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Western Blotting Western blot analysis in human cell line HEL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | MNYLEVNRRVNNFGSGLTALGLKPK |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | O60488  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | ACSL4 (acyl-CoA synthetase long-chain family member 4) has a higher affinity for arachidonic acid (AA) and eicosapentanoic acid (EPA), as opposed to other isoforms. Thus, it plays an essential role in AA metabolism. It is responsible for the de novo synthesis of diacylglycerol (DAG). ACSL4 also mediates the storage of fatty acids in the form of triacylglycerol. Two point mutations in this gene are linked with nonspecific mental retardation. ACSL4 expression is up-regulated in liver, colon and aggressive forms of breast cancer. It has potential as a marker for aggressive phenotype of breast cancer. This gene is linked to metabolic syndrome, as a mutation in ACSL4 gene leads to abnormal fatty acid composition of phosphatidylcholines present in plasma membrane. Inactivation of this gene is found in elliptocytosis, Alport syndrome, and mental retardation. Therefore, it is suggested that FACL4 regulates intellectual capability and coordination skills, through FACL4-derived arachidonoyl phospholipids. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | ACSL4 (acyl-CoA synthetase long-chain family member 4) belongs to the family of mammalian long chain acyl-CoA synthetases. This family consists of five isoforms, one being ACSL4. It is localized to the endoplasmic reticulum (ER) associated with mitochondria, and peroxisome. It is expressed in smooth muscle and neuronal cells. However, its predominant expression is in steroidogenic tissues. It is highly expressed in spleen, placenta, testis, ovary, brain and adrenal cortex. ACSL4 gene is localized to human chromosome Xq23. The encoded protein has a molecular weight of 75kDa. |
| Immunogen: | Long-chain-fatty-acid–CoA ligase 4 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST74278 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |