Anti-BAZ1A antibody produced in rabbit
SIGMA/HPA002730 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-ATP-dependent chromatin remodelling protein antibody produced in rabbit; Anti-ATP-utilizing chromatin assembly and remodeling factor 1 antibody produced in rabbit; Anti-Bromodomain adjacent to zinc finger domain protein 1A antibody produced in rabbit; Anti-Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody produced in rabbit; Anti-hACF1 antibody produced in rabbit
Product Type: Chemical
Western Blotting JOURNAL CITATION: Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. By: Dowdle, J. A., Mehta, M., et al. in PLoS Genet, 2013. PubMed ID: 24244200 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003945), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Mouse BAZ1A (ACF1) is dispensable for double-strand break repair but is essential for averting improper gene expression during spermatogenesis. By: Dowdle, J. A., Mehta, M., et al. in PLoS Genet, 2013. PubMed ID: 24244200 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003945), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and pancreas tissues using HPA002730 antibody. Corresponding BAZ1A RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong nuclear positivity in syncytiotrophoblast.
Immunohistochemistry Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RGHRESALKETLLQEKSRICAQLAR |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:20-1:50 |
| UniProt accession no. | Q9NRL2  |
| Application: | Anti-BAZ1A antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | BAZ1A (bromodomain adjacent to zinc finger domain 1A) is involved in the ATP-dependent ′nucleosome remodelling′ activity. It is a subunit of Drosophila ACF (ATP-dependent chromatin assembly factor large subunit). In Drosophila, BAZ1A forms a heteromer chromatin remodelling complex with the nucleosome-dependent ATPase ISWI (imitation swi). This complex has the ability to bind naked DNA but not nucleosomal DNA. It catalyzes the process of transfering histone octamers between DNA segments. The BAZ1A-ISWI heteromer also interacts with small histone fold proteins CHRAC (chromatin accessibility complex)-14 and CHRAC-16 during nucleosome remodelling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Bromodomain adjacent to zinc finger domain protein 1A recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST70546 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |