Anti-AHSG antibody produced in rabbit
SIGMA/HPA001524 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-α-2-HS-glycoprotein precursor antibody produced in rabbit; Anti-α-2-Z-globulin antibody produced in rabbit; Anti-Ba-α-2-Glycoprotein antibody produced in rabbit; Anti-Fetuin-A antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human bone marrow, cerebral cortex, liver and lymph node using Anti-AHSG antibody HPA001524 (A) shows similar protein distribution across tissues to independent antibody HPA001525 (B).
Enhanced Validation-RNAi Knockdown Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probes 1 and 2, using Anti-AHSG antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry Immunohistochemical staining of human stomach shows moderate positivity in extracellular matrix.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate extra-cellular positivity and moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-AHSG antibody HPA001524.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-AHSG antibody HPA001524.
Immunohistochemistry Immunohistochemical staining of human bone marrow using Anti-AHSG antibody HPA001524.
Immunohistochemistry Immunohistochemical staining of human lymph node using Anti-AHSG antibody HPA001524.
Immunofluorescence Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent RNAi knockdown independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LPPSTYVEFTVSGTDCVAKEATEAA |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | P02765  |
| Application: | Anti-AHSG antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | AHSG (α-2-HS-glycoprotein) gene encodes a glycoprotein that is present in the serum. It is synthesized by hepatocytes. The gene is localized to human chromosome 3q27, a susceptibility locus for type 2 diabetes and the metabolic syndrome. It may be involved in the regulation of postprandial glucose disposal, insulin sensitivity, weight gain, and fat accumulation. It may serve as a potential therapeutic target in the treatment of type 2 diabetes, obesity, and other insulin-resistant conditions. It is an antagonist of transforming growth factor β, regulating cytokine-dependent osteogenesis. It inhibits insulin receptor tyrosine kinase and some protease activities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | α-2-HS-glycoprotein precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST84500 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |