Anti-ACO2 antibody produced in rabbit
SIGMA/HPA001097 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Aconitase antibody produced in rabbit; Anti-Aconitate hydratase, mitochondrial precursor antibody produced in rabbit; Anti-Citrate hydro-lyase antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue. By: Ilgen, P., Stoldt, S., et al. in PLoS One, 2014. PubMed ID: 25025184 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0101563), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human heart muscle and liver tissues using HPA001097 antibody. Corresponding ACO2 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry Immunohistochemical staining of human duodenum shows strong strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human liver shows very weak positivity in hepatocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Western Blotting Western blot analysis in human cell line HL-60.
Western Blotting Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GKKFRLEAPDADELPKGEFDPGQDT |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:500-1:1000 | |
| UniProt accession no. | Q99798  |
| Application: | Anti-ACO2 antibody produced in rabbit has been used for protein arrary analysis. Anti-ACO2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | Aconitase (ACO2) is an important enzyme located in the mitochondria and catalyzes the interconversion of citrate and isocitrate in the tricarboxylic acid cycle. The expression of this gene may be altered in certain types of cancer. It may play a crutial role in gastric cancer and may potentially serve as a prognostic biomarker. Defect in mitochondrial aconitase is found to be associated with an infantile neurodegenerative disorder affecting mainly the cerebellum and retina. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The ACO2 (aconitase 2) gene is mapped to human chromosome 22q13.2. |
| Immunogen: | Aconitate hydratase, mitochondrial precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST73402 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |