Anti-SH3BP1 antibody produced in rabbit
SIGMA/HPA000757 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-3BP-1; Anti-PDXP antibody produced in rabbit; Anti-PLP phosphatase; Anti-SH3 domain-binding protein 1; Anti-SH3BP1
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human spleen and kidney tissues using Anti-SH3BP1 antibody. Corresponding SH3BP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human spleen shows high expression.
Immunohistochemistry Immunohistochemical staining of human kidney shows low expression as expected.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ENLSNLRYLMKFLARLAEEQEVNKM |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:20-1:50 | |
| UniProt accession no. | Q96GD0  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | The gene SH3BP1 is also known as CIN and PLP. It belongs to a unique cofilin-activating phosphatase of the haloacid dehalogenase (HAD) superfamily. The gene directly dephosphorylates cofilin with high specificity and colocalizes with cofilin in motile and dividing cells. Loss of this gene activity blocks phosphocycling of cofilin, stabilizes F-actin structures and causes massive cell division defects. CIN is an important novel regulator of cofilin-mediated actin reorganization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Pyridoxal phosphate phosphatase recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST73373 . |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |