Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse
SIGMA/F9291 - clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | biotin conjugate |
| form | buffered aqueous glycerol solution |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | dot blot: suitable (chemiluminescent detection) |
| Application: | Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host. Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes. Browse additional application references in our FLAG® Literature portal. |
| General description: | The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins. |
| Legal Information: | ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide |
| Preparation Note: | Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL. |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |