Anti-BrdU antibody, Mouse monoclonal
SIGMA/B8434 - clone BU-33, purified from hybridoma cell culture
Synonym: Monoclonal Anti-BrdU antibody produced in mouse; Anti Brdu; Brdu Antibody; Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal; Anti-
MDL Number: MFCD00164550
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways. By: Feng, C., Zhang, Y., et al. in Oxid Med Cell Longev, 2017. PubMed ID: 29147462 Image collected and cropped by CiteAb from the following publication, (https://www.hindawi.com/journals/omcl/2017/7426458/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways. By: Feng, C., Zhang, Y., et al. in Oxid Med Cell Longev, 2017. PubMed ID: 29147462 Image collected and cropped by CiteAb from the following publication, (https://www.hindawi.com/journals/omcl/2017/7426458/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Oxygen-Sensing Nox4 Generates Genotoxic ROS to Induce Premature Senescence of Nucleus Pulposus Cells through MAPK and NF-κB Pathways. By: Feng, C., Zhang, Y., et al. in Oxid Med Cell Longev, 2017. PubMed ID: 29147462 Image collected and cropped by CiteAb from the following publication, (https://www.hindawi.com/journals/omcl/2017/7426458/), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat intestin, BrdU treated (in vivo) sections with 1 μg/mL Monoclonal Anti-BrdU, Clone: BU-33 (Cat. No. B8434) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | purified from hybridoma cell culture |
| purified immunoglobulin | |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BU-33, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | wide range |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU |
| Application: | Anti-BrdU antibody has been used in: • BrdU labeling • immunohistochemistry • immunostaining • BrdU assay |
| Application: | Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody: • to detect 5-fluorouridine-labelled RNA • to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells • against BrdU labelled rat retinal sections |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | 5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle. Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo. |
| Immunogen: | bromodeoxyuridine conjugated to KLH. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide. |
| Specificity: | Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |