Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal
SIGMA/A7732 - clone EA-53, purified from hybridoma cell culture
Synonym: Anti-ACTN2; Anti-Actinin α2; Anti-CMD1AA; Anti-MGC107582; Anti-1110008F24Rik; Sarcomeric Alpha Actinin Antibody; Sarcomeric Alpha Actinin Antibody - Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal
Product Type: Chemical
Flow cytometry/Cell sorting JOURNAL CITATION: Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes. By: Kim, H. S., Yoon, J. W., et al. in Sci Rep, 2017. PubMed ID: 29062050 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-14198-y), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes. By: Kim, H. S., Yoon, J. W., et al. in Sci Rep, 2017. PubMed ID: 29062050 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-14198-y), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Functional expression and pharmaceutical efficacy of cardiac-specific ion channels in human embryonic stem cell-derived cardiomyocytes. By: Kim, H. S., Yoon, J. W., et al. in Sci Rep, 2017. PubMed ID: 29062050 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41598-017-14198-y), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human tongue with 12 μg/mL Monoclonal Anti-α-Actinin (Sarcomeric) Clone: EA-53 (Cat. No. A7732) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-α-Actinin (Sarcomeric) Clone: EA-53 (Cat. No. A7732). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Mouse heart 2. Mouse skeletal muscle 3. C2C12 differentiated cells 4. Rat heart 5. Rat skeletal muscle
Western Blotting Rat skeletal muscle extract were separated on SDS-PAGE and probed with Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal, Clone: EA-53 (Cat. No. A7732). The antibody was developed using 1:4,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:2,000 antibody 4. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | EA-53, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using protease-digested sections of human tongue. |
| western blot: 2-4 μg/mL using rat skeletal muscle extract | |
| UniProt accession no. | Q08043  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Immunofluorescence (2 papers) Immunohistochemistry (1 paper) |
| Application: | Mouse Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody has been used for immunocytochemical (1:800) and immunohistochemical (1:100) applications. |
| Application: | Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody. |
| Biochem/physiol Actions: | α-Actinin maintains the structure of cytoskeletal organization and regulates muscle contraction. α-Actinin functions as an actin crosslinker and promotes cell migration. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-Actinin belongs to the spectrin protein superfamily. α-Actinin dimerizes in an antiparallel fashion via interaction of the central rod domains. Four isoforms are found in human and mice. α-actinin-1 is ubiquitously expressed and located primarily in focal adhesions, whereas α-actinin-4 is present in certain membrane ruffles and seems to play a role in endocytosis and tumor cell motility. ACTN1 (α-actinin-1) and ACTN4 (α-actinin-4) encodes non-muscle proteins, while ACTN2 (α-actinin-2) and ACTN3 (α-actinin-3) encodes muscle proteins. Monoclonal Anti-α-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish α-actinin. |
| Immunogen: | Purified rabbit skeletal α actinin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-α-Actinin (Sarcomeric) specifically recognizes α-skeletal muscle actinin and α-cardiac muscle actinin. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |