Anti-Human Albumin antibody produced in rabbit
SIGMA/A3293 - whole antiserum
Synonym: Albumin Antibody; Albumin Antibody - Anti-Human Albumin antibody produced in rabbit; Anti Human Albumin Antibody
MDL Number: MFCD00162390
Product Type: Chemical
ELISA Anti-Human Albumin antibody produced in Rabbit (Cat. No. A3293), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Albumin by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| mol wt | antigen 66.437-66.6 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:60,000 |
| quantitative precipitin assay: 2.4-3.6 | |
| UniProt accession no. | P02768  |
| Application: | Anti-Human Albumin antibody has been used in enzyme linked immunosorbent assay (ELISA) and in immobilization of antibodies for human serum albumin (HSA). |
| Application: | Anti-human albumin antibody may be used in quantitative precipitin assay, high-throughput protein microarray analysis and immunocytochemistry . |
| Application: | Rat hepatocytes were grown on collagen-coated coverslips, fixed in formalin and permeablized with methanol prior to incubation with rabbit anti-human albumin antibody. |
| Biochem/physiol Actions: | Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions among several others . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . |
| Biochem/physiol Actions: | Albumin plays a major role in transportation and deposition of many exogenous and endogenous substances in blood. |
| Biochem/physiol Actions: | Anti-human albumin antibodies are specific for human albumin. |
| General description: | Albumin′s primary structure is composed of a single chain of amino acids and the secondary structure is composed of α-helix of six turns and 17 disulfide bridges. The tertiary structure is 3D and is composed of three domains I II and III. Each of these domains are made of sub-domains IAB, IC, IIAB, IIC, IIIAB, IIIC, respectively. |
| Immunogen: | Human albumin |
| Physical form: | Supplied as a liquid containing 15mM sodium azide as preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |