Anti-Actin antibody produced in rabbit
SIGMA/A2066 - affinity isolated antibody, buffered aqueous solution
Synonym: Actin Antibody Sigma; Anti Actin; Anti Actin Antibody Western Blot; Anti Actin Antibody for western blot - Anti-Actin antibody produced in rabbit; Anti Actin Sigma; Sigma Actin Antibody
MDL Number: MFCD00145889
Product Type: Chemical
Western Blotting JOURNAL CITATION: The inhibition of the highly expressed miR-221 and miR-222 impairs the growth of prostate carcinoma xenografts in mice. By: Mercatelli, N., Coppola, V., et al. in PLoS One, 2008. PubMed ID: 19107213 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0004029), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Rb induces a proliferative arrest and curtails Brn-2 expression in retinoblastoma cells. By: Cobrinik, D., Francis, R. O., et al. in Mol Cancer, 2006. PubMed ID: 17163992 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-5-72), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Rb induces a proliferative arrest and curtails Brn-2 expression in retinoblastoma cells. By: Cobrinik, D., Francis, R. O., et al. in Mol Cancer, 2006. PubMed ID: 17163992 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-5-72), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluorescence. CFB cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:40 Anti-Actin antibody produced in Rabbit (Cat. No. A2066) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887), and 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody (Cat. No. F9887). Results Two Anti-Actin antibodies, A2066 (A) and A2103 (B) target different regions of Actin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry
Staining of formalin-fixed, paraffin-embedded section of human appendix using Rabbit Anti-Actin (Cat. No. A2066).
Antibody dilution 1:50
Staining of formalin-fixed, paraffin-embedded section of human appendix using Rabbit Anti-Actin (Cat. No. A2066).
Antibody dilution 1:50
Immunofluorescence Chicken fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:40 Rabbit Anti-actin (Cat. No. A2066). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate (Cat. No. F9887).
Immunoblotting
Chicken gizzard whole cell extracts were separated on SDS-PAGE, blotted with Rabbit Anti-Actin (Cat. No. A2066) and developed using Goat Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and AEC substrate. (Lane A) Antibody dilution 1:200; (Lane B) Negative control (only secondary antibody).
Chicken gizzard whole cell extracts were separated on SDS-PAGE, blotted with Rabbit Anti-Actin (Cat. No. A2066) and developed using Goat Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and AEC substrate. (Lane A) Antibody dilution 1:200; (Lane B) Negative control (only secondary antibody).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:100 Rabbit Anti-Actin (Cat. No. A2066). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. P19 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 42 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | wide range, vertebrates, human, slime mold, amoeba, chicken |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections |
| indirect immunofluorescence: 1:40 using chicken fibroblasts | |
| western blot: 1:100 using chicken gizzard extract | |
| UniProt accession no. | P68133  |
| Application: | May be used to study actin structure and function and to probe binding sites of actin-binding proteins. |
| Application: | Rabbit anti-actin antibody can be used for western blot assays. The antibody can also be used for immunohistochemistry (1:25, using human or animal tissue sections) and indirect immunofluorescence (1:40, using chicken fibroblasts) applications. Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates. Actin was detected at 42 kDa. |
| Application: | Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa. |
| Biochem/physiol Actions: | Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. Actin protein is essential for the formation of mature platelets. Mutations in ACTA1 (actin, α 1) is known to cause thin filament myopathy such as nemaline myopathy, congenital myopathy, actin myopathy, intranuclear rod myopathy, cap myopathy and core myopathy. Upregulation of the gene is observed in orthotopic liver transplantation without any earlier evidence of autoimmune liver disease. The encoded protein actin forms a network of filaments and is responsible for the shape of the cell. It provides cellular strength and promotes active movement of the cell. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The ACTA1 (actin, α 1) gene is mapped to human chromosome 1q42.13. The gene codes for actin 1, a thin filament component of skeletal muscle. In immunoblotting, the product localizes actin in many species ranging from human skeletal muscle to amoeba. The product recognizes the 42 kDa actin band using immunoblotting techniques in human or animal tissue extracts. |
| Immunogen: | The C-terminal actin fragment attached to Multiple Antigen Peptide (MAP) backbone. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as preservative. |
| Specificity: | Anti-Actin specifically stains typical stress fibers in cultured chicken fibroblasts. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |