Certain types of PCR, such as RAPD PCR, require high concentrations of Taq DNA Polymerase. However, adding larger volumes of Taq DNA Pol will increase the glycerol concentration in the reaction mix (Taq DNA Polymerase storage buffer contains 50% glycerol to prevent freezing) and high glycerol levels can interfere with PCR. To avoid inadvertently raising glycerol concentrations in the final mix, Taq DNA Polymerase at 15 U/µLconcentration is recommended.
10X incubation buffers, one with and one without MgCl2, are provided.
A solution of 25mM MgCl2 is also supplied.