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Q-BioTaq™ DNA Polymerase

By MP Biomedicals

For multiplex PCR and RAPDs

  • Recombinant, truncated form of Thermus aquaticus
  • Highly thermostable (98°C)
  • No 5'-3' exonuclease activity
  • Enzyme cannot degrade the 5' end of primers

Q-BioTaq™ DNA Polymerase is a thermostable DNA polymerase which can be used effectively for PCR and DNA sequencing by the chain termination method. This enzyme is encoded by a modified form of the Thermus aquaticus DNA polymerase gene with an N-terminal deletion. The properties of Q-BioTaq DNA Polymerase include high thermostability and absence of 5'-3' exonuclease activity. The absence of 5'-3' exonuclease activity and high thermostability of this enzyme make it particularly useful in applications where multiple amplifications are required. The enzyme is highly purified and free of RNases and endo- and exonucleases.

Unit definition: One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions.
Storage: 25 ml Tris HCl (pH 8.0 at 25°C); 100 mM NaCl; 0.1 mM EDTA; 1 mMDTT; 0.75% Triton; 50% gycerol
Incubation Buffer 1x: 67 mM Tris HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2 SO4; 0.01% Tween 20; 3.5 mM MgCl2
Quality Control: Activity, SDS-PAGE/purity, nuclease, specific PCR on genomic and phage templates.

* The concentration of Q-BioTaq, 5 U/µL, is in Klentaq units. 1U Klentaq = 4U DNA Taq pol.

Amplification of a 400 bp fragment of human β globin with Q-BioTaq™ DNA polymerase Varying amounts of human β globin, 0.6ng to 100ng, were amplified under the following conditions: 50µl reaction; 50 pmoles each specific oligo, 300µM each dNTP, 0.5U Q-BioTaq, 1 x Q-BioTaq Buffer; 5' at 93°C then 1' at 91°C, 1' at 62°C, 2' at 70°C for 37 cycles, 1' at 91°C, 1' at 62°C and 10' at 70°C for 1 cycle. Lane 1 marker pBR322/HaeIII, Lane 2. 0.6ng, Lane 3. 10ng, Lane 4. 100ng of template DNA, respectively.

Storage: -20°C

Two different 10X incubation buffers are provided, one with and one without MgCl2, MgCl2 at 25mM is supplied in a separate tube.

             
Cat.No. Name Size Conc. EA    
17-EPQBT010 Q-BioTaq 100 U 5 U/µL* $126.00
1/EA
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17-EPQBT015 Q-BioTaq 5 x 100 U 5 U/µL* $544.50
1/EA
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17-EPQBT025 Q-BioTaq 250 U 5 U/µL* $297.00
1/EA
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17-EPQBT050 Q-BioTaq 500 U 5 U/µL* $470.25
1/EA
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17-EPQBT100 Q-BioTaq 1000 U 5 U/µL* $979.88
1/EA
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