For multiplex PCR and RAPDs
- Recombinant, truncated form of Thermus aquaticus
- Highly thermostable (98°C)
- No 5'-3' exonuclease activity
- Enzyme cannot degrade the 5' end of primers
Q-BioTaq™ DNA Polymerase is a thermostable DNA polymerase which can be used effectively for PCR and DNA sequencing by the chain termination method. This enzyme is encoded by a modified form of the Thermus aquaticus DNA polymerase gene with an N-terminal deletion. The properties of Q-BioTaq DNA Polymerase include high thermostability and absence of 5'-3' exonuclease activity. The absence of 5'-3' exonuclease activity and high thermostability of this enzyme make it particularly useful in applications where multiple amplifications are required. The enzyme is highly purified and free of RNases and endo- and exonucleases.
|Unit definition:||One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions.|
|Storage:||25 ml Tris HCl (pH 8.0 at 25°C); 100 mM NaCl; 0.1 mM EDTA; 1 mMDTT; 0.75% Triton; 50% gycerol|
|Incubation Buffer 1x:||67 mM Tris HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2 SO4; 0.01% Tween 20; 3.5 mM MgCl2|
|Quality Control:||Activity, SDS-PAGE/purity, nuclease, specific PCR on genomic and phage templates.|
* The concentration of Q-BioTaq, 5 U/µL, is in Klentaq units. 1U Klentaq = 4U DNA Taq pol.
Amplification of a 400 bp fragment of human β globin with Q-BioTaq™ DNA polymerase Varying amounts of human β globin, 0.6ng to 100ng, were amplified under the following conditions: 50µl reaction; 50 pmoles each specific oligo, 300µM each dNTP, 0.5U Q-BioTaq, 1 x Q-BioTaq Buffer; 5' at 93°C then 1' at 91°C, 1' at 62°C, 2' at 70°C for 37 cycles, 1' at 91°C, 1' at 62°C and 10' at 70°C for 1 cycle. Lane 1 marker pBR322/HaeIII, Lane 2. 0.6ng, Lane 3. 10ng, Lane 4. 100ng of template DNA, respectively.
Two different 10X incubation buffers are provided, one with and one without MgCl2, MgCl2 at 25mM is supplied in a separate tube.