Q-BioTaq™ DNA Polymerase
By MP BiomedicalsFor multiplex PCR and RAPDs
- Recombinant, truncated form of Thermus aquaticus
- Highly thermostable (98°C)
- No 5'-3' exonuclease activity
- Enzyme cannot degrade the 5' end of primers
Q-BioTaq™ DNA Polymerase is a thermostable DNA polymerase which can be used effectively for PCR and DNA sequencing by the chain termination method. This enzyme is encoded by a modified form of the Thermus aquaticus DNA polymerase gene with an N-terminal deletion. The properties of Q-BioTaq DNA Polymerase include high thermostability and absence of 5'-3' exonuclease activity. The absence of 5'-3' exonuclease activity and high thermostability of this enzyme make it particularly useful in applications where multiple amplifications are required. The enzyme is highly purified and free of RNases and endo- and exonucleases.
| Unit definition: | One unit is the amount of enzyme required to catalyse the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74°C under assay conditions. |
| Storage: | 25 ml Tris HCl (pH 8.0 at 25°C); 100 mM NaCl; 0.1 mM EDTA; 1 mMDTT; 0.75% Triton; 50% gycerol |
| Incubation Buffer 1x: | 67 mM Tris HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2 SO4; 0.01% Tween 20; 3.5 mM MgCl2 |
| Quality Control: | Activity, SDS-PAGE/purity, nuclease, specific PCR on genomic and phage templates. |
* The concentration of Q-BioTaq, 5 U/µL, is in Klentaq units. 1U Klentaq = 4U DNA Taq pol.
Amplification of a 400 bp fragment of human β globin with Q-BioTaq™ DNA polymerase Varying amounts of human β globin, 0.6ng to 100ng, were amplified under the following conditions: 50µl reaction; 50 pmoles each specific oligo, 300µM each dNTP, 0.5U Q-BioTaq, 1 x Q-BioTaq Buffer; 5' at 93°C then 1' at 91°C, 1' at 62°C, 2' at 70°C for 37 cycles, 1' at 91°C, 1' at 62°C and 10' at 70°C for 1 cycle. Lane 1 marker pBR322/HaeIII, Lane 2. 0.6ng, Lane 3. 10ng, Lane 4. 100ng of template DNA, respectively.
Storage: -20°C
Two different 10X incubation buffers are provided, one with and one without MgCl2, MgCl2 at 25mM is supplied in a separate tube.