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Taq DNA Polymerase

By MP Biomedicals

Recombinant form - for general PCR applications.

  • Highly reproducible results
  • Ideal for T/A cloning

Highly processive, recombinant, thermostable 5'-3' DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain. Remains functional even after prolonged incubation at 95°C. Applications include PCR, RT-PCR, DNA sequencing and DNA labeling. This enzyme possesses 5'-3' exonucleases activiy.

Unit definition: One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid-insoluble material in 30 min. at 74°C under assay conditions.
Storage conditions: 20mM Tris-HCl pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM dithiothreitol, 0.5% Tween 20, 0.5% Nonidet P40, 50% glycerol. Stored at -20°C
Incubation buffer with MgCl2 (1X): 10mM Tris-HCl pH 9.0, 50mM KCl, 1.5mM MgCl2, 0.1% Triton X-100, 0.2 mg/ml BSA
Incubation buffer w/o MgCl2 (1X): 10mM Tris-HCl pH 9.0, 50mM KCl, 0.1% Triton X100, 0.2 mg/ml BSA
Quality control: Performed on every lot of enzyme: Specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.

Storage: -20°C

               
Cat.No. Name Size Conc. Incubation Buffers Incl. EA    
17-EPTQA025 Taq DNA Polymerase & Buffer 250 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $98.10
1/EA
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17-EPTQA100 Taq DNA Polymerase & Buffer 1000 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $318.35
1/EA
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17-EPTQA105 Taq DNA Polymerase & Buffer 5 x 1000 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $1532.85
1/EA
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17-EPTQA325 Taq DNA Polymerase & Buffer 3 x 250 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $275.25
1/EA
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17-EPTQA500 Taq DNA Polymerase & Buffer 5000 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $2043.10
1/EA
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17-EPTQA925 Taq DNA Polymerase & Buffer 10 x 250 U 5 U/µL 10x Buffer with MgCl2; 10x Buffer w/o MgCl2; 25mM MgCl2 sol. $882.90
1/EA
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17-EPTQBUF1 10x Buffer with MgCl2 5 x 1mL     $35.75
1/EA
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17-EPTQBUF2 10x Buffer w/o MgCl2; 25mM MgCl2 sol. 5 x 1mL     $35.75
1/EA
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17-EPTQD025 Taq DNA Polymerase & Buffer 250 U 5 U/µL only 10x Buffer with MgCl2 $94.10
1/EA
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17-EPTQD325 Taq DNA Polymerase & Buffer 3 x 250 U 5 U/µL only 10x Buffer with MgCl2 $268.45
1/EA
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17-EPTQD925 Taq DNA Polymerase & Buffer 10 x 250 U 5 U/µL only 10x Buffer with MgCl2 $869.30
1/EA
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