Recombinant form - for general PCR applications.
- Highly reproducible results
- Ideal for T/A cloning
Highly processive, recombinant, thermostable 5'-3' DNA polymerase catalyzes the addition of mononucleotide units to the 3'-OH end of a primer chain. Remains functional even after prolonged incubation at 95°C. Applications include PCR, RT-PCR, DNA sequencing and DNA labeling. This enzyme possesses 5'-3' exonucleases activiy.
|Unit definition:||One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid-insoluble material in 30 min. at 74°C under assay conditions.|
|Storage conditions:||20mM Tris-HCl pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM dithiothreitol, 0.5% Tween 20, 0.5% Nonidet P40, 50% glycerol. Stored at -20°C|
|Incubation buffer with MgCl2 (1X):||10mM Tris-HCl pH 9.0, 50mM KCl, 1.5mM MgCl2, 0.1% Triton X-100, 0.2 mg/ml BSA|
|Incubation buffer w/o MgCl2 (1X):||10mM Tris-HCl pH 9.0, 50mM KCl, 0.1% Triton X100, 0.2 mg/ml BSA|
|Quality control:||Performed on every lot of enzyme: Specific PCR on genomic and phage templates, activity check, absence of ribonucleases, absence of nickases and endonucleases, absence of 3' exonucleases, purity on SDS-PAGE, absence of proteases.|