Advanced Search

Acrylamide/Bis-Acrylamide Solutions 40% (w/v)

By MP Biomedicals

Ultrapure grade, prepared from highly purified acrylamide (4X) and bis-acrylamide (2X) using deionized ultra-filtered water for electrophoresis of proteins and nucleic acids.

Final ratio:

  • 37.5:1 for the separation of high molecular weight proteins
  • 29:1 for sequencing gels and the separation of proteins
  • 19:1 Optimal for sequencing gels and also for the separation of peptides

Polyacrylamide is a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Polyacrylamide gels are prepared by free radical dependent co-polymerization of acrylamide and a cross-linker such as N, N-methylene-bis-acrylamide (Bis). The acrylamide to cross-linker ratio determines the pore size of the gel, which in turn determines the size range of molecules that can be separated (e.g. proteins are generally separated using ratios ranging from 29:1 to 37.5:1 whereas the a ratio of 19:1 is commonly used for DNA sequencing gels or separation of peptides).

Reliable electrophoretic experimental results demand high purity reagents with low levels of contaminants. Impurities may impair homogeneity and reproducibility of gel polymerization. Moreover, charged impurities may exert a major effect on electrophoretic separation. Qbiogene's pre-mixed acrylamide solutions are prepared from Ultrapure Molecular Biology Grade reagents ensuring uniform polymerization upon addition of ammonium persulfate and N, N, N, N - tetramethylenediamine (TEMED).

For greater convenience and safety, Qbiogene offers ready-to-use premixed, pre-filtered acrylamide/bis-acrylamide 40% (w/v) stock solutions prepared with 4X crystallized acrylamide and deionized, ultrafiltered water. Ready-to-use acrylamide solutions eliminate the risks associated with the handling of acrylamide powder. The solutions are packaged in UV-proof glass bottles.


UN code: UN2074
Conductivity (40% solution in H2O, 25°C): ≤ 5µS/cm
Acrylic acid (titrimetry): ≤ 0.001%
A290 (1% in H2O): ≤ 0.1
Acrylamide powder purified by recrystallization 4X
Bis-acrylamide powder purified by recrystallization 2X
Heavy metals (as Pb): < 0.0002%
Polymerization time: < 30 min
RNAse, DNAse, Protease free

Storage: +4°C protected from light
Risk and Safety: Hazardous material Please refer to the material safety data sheet for proper handling

Cat.No. Name Size Final Ratio EA    
17-BIAC1902 Acrylamide/BisAcrylamide, 40% solution 1L 19:1 DISCONTINUED  
17-BIAC1903 Acrylamide/BisAcrylamide, 40% solution 3 x 1L 19:1 $0.00
Add To Favorites
17-BIAC2902 Acrylamide/BisAcrylamide, 40% solution 1L 29:1 $356.35
Add To Favorites
17-BIAC2903 Acrylamide/BisAcrylamide, 40% solution 3 x 1L 29:1 $0.00
Add To Favorites
17-BIAC3752 Acrylamide/BisAcrylamide, 40% solution 1L 37.5:1 $404.84
Add To Favorites
17-BIAC3753 Acrylamide/BisAcrylamide, 40% solution 3 x 1L 37.5:1 $0.00
Add To Favorites

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price

Request Literature

Please fill out the form below if you want to request literature from Krackeler Scientific.


Quick Order

* Required

New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top