- No Heat Shock Required
- Transform-It™ Solution is Ready-to-Use
- Transform Plasmids, Ligations, and Cosmids
- Store Competent Cells 10 Weeks or Longer
- More Reliable than "Do-it-Yourself" Methods
- Easy Procedure with Minimal Hands-On Time
The Transform & Grow™ Bacterial Transformation Kit provides a simple and efficient method for the preparation, transformation and storage of competent E. coli cells. Bacterial cells are grown to mid-log phase at 37°C, pelleted and resuspended in Transform-It Solution. This unique buffer enables the cells to become immediately available for transformation and permits freezing the cells for future use without further preparation. The competent cells remain stable at -70°C for at least 10 weeks. The Transform & Grow Bacterial Transformation Kit eliminates the requirement for heat shock and prepares competent cells within two and a half hours with only 30 minutes of hands-on manipulation. The kit includes Transform-It Solution, SUPER-COMP Media and Roll & Grow® Plating Beads to prepare competent cells with a reliable and reproducible transformation efficiency of 2-8 x 106 transformants per µg of supercoiled plasmid DNA. Reagents for 50 transformations are provided.
Kit Components: Transform-It Solution, SUPER-COMP Media, Roll & Grow Plating Beads