High efficiency transformation of yeast cells
Transformation process complete in less than 90 minutes with efficiencies routinely greater than 104 transformants per µg plasmid DNA.
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two-hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation efficiencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
Kit Components: TE (pH 7.5), Lithium/Cesium Acetate, Carrier DNA, Histamine, PEG, TE/Cation MIXX, SOS