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Alkali-Cation™ Yeast Transformation Kit

By MP Biomedicals

High efficiency transformation of yeast cells

Alkali-Cation™ Yeast Transformation Kit

Transformation process complete in less than 90 minutes with efficiencies routinely greater than 104 transformants per µg plasmid DNA.

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two-hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation efficiencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

Kit Components: TE (pH 7.5), Lithium/Cesium Acetate, Carrier DNA, Histamine, PEG, TE/Cation MIXX, SOS

Cat.No. Name Size EA    
17-2200-200 Alkali-Cation Yeast Transf Kit 250 preps $346.50
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