For anion exchange separation of carbohydrates
RCX-10 HPLC columns are designed for the isocratic or gradient separation of carbohydrates. Simple samples with two or three carbohydrates can be separated isocratically. Complex samples require gradient elution to fully resolve all the oligomers.
The separation of carbohydrates at basic pH is possible because each carbohydrate carries a different negative charge at basic pH. Use a variety of detectors (refractive index, ultraviolet, and conductivity) for isocratic separations. A pulsed amperometric detector (PAD) allows you to perform gradient separations.