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By Sartorius

Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications. The AdenoPACK adenovirus purification and concentration kits offer researchers who need to recover up to 3 x 1013 purified recombinant adenovirus particles for in-vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only 2 hours. These straight forward kits replace time-consuming and labor-intensive 48 hour CsCl density gradients.

AdenoPACK 20

Vivapure AdenoPACK 20 is the small scale kit in the AdenoPACK series, purifying up to 1 x 1012 viral particles from 20mL cell culture. Especially when testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method.

Kit includes:

  • 6 AdenoPACK Maxi spin columns
  • 120µL Benzonase® Nuclease 12.5 U/µL
  • 6 Vivaclear Maxi membranes 0.45µm
  • 6 Empty 50mL tubes
  • Loading Buffer (10x) 1 x 25mL
  • Washing Buffer (10x) 1 x 30mL
  • Elution Buffer 1 x 20mL
  • 6 Vivaspin 20 concentrators, 100 kDa MWCO
Cat.No. Name EA    
109-VSAVPQ020 AdenoPACK 20 $388.00
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AdenoPACK 100

Vivapure AdenoPACK 100 is optimally suited for adenovirus purification from up to 200mL cell culture for in vitro transfection. This kit contains two AdenoPACK 100 units, which can be either used in tandem for the purification of up to 200mL cell culture supernatant or individually for purifying virus from up to 60mL cell culture.

Kit includes:

  • 2 AdenoPACK Membrane Adsorbers
  • 200µL Benzonase® Nuclease* 12.5 U/µL
  • 2 x 50mL Syringes
  • 4 x Minisart Plus
  • 4 x 20mL Syringe
  • 2 Tubing sets
  • 2 x 10mL syringe (elution)
  • 2 Elution Tips (Red)
  • 1 x 25mL Loading Buffer (10x)
  • 1 x 120mL Washing Buffer
  • 1 x 20mL Elution Buffer
  • 4 Vivaspin 20 concentrators
Cat.No. Name EA    
109-VSAVPQ101 AdenoPPACK 100 $698.00
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AdenoPACK 500

Vivapure AdenoPACK 500 is the direct upscale kit to the AdenoPACK 100, for adenovirus purification. In only 2 hours up to 3 x 1013 adenovirus particles are purified and concentrated from 500mL cell culture. This completely ready-to-use kit is conveniently operated by a laboratory pump, offering optimal flow control and minimal hands-on time.

Kit includes:

  • 1 AdenoPACK Membrane Adsorber
  • 500µL Benzonase® Nuclease* 12.5 U/µL
  • 1 x Sartopore 2 150
  • 2 Tubing sets
  • 1 x 10mL syringe (elution)
  • 1 x 60mL Loading Buffer (10x)
  • 1 x 30mL Washing Buffer
  • 1 x 20mL Elution Buffer
  • 2 Vivaspin 20 concentrator, 100 kDa MWCO
Cat.No. Name EA    
109-VSAVPQ501 AdenoPACK 500 $1520.00
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