Advanced Search

Corning® Nonenzymatic Cell Dissociation Agents

By Corning® cellgro®
Corning® Nonenzymatic Cell Dissociation Agents

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators which gently dislodges adherent cells in tissue culture. It is designed to be an alternative to trypsin when used in conjunction with serum-free or serum-containing media. Cells can be exposed to Cellstripper® for longer periods of time without the risk of damage associated with protein digestive enzymes like trypsin. Experimental comparisons of dissociation solutions for cell detachment reveal that Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

The following instructions are applicable to most cell lines. Actual procedures and concentrations should be determined by experience with individual cell lines. Researchers should regularly monitor cell viability at sub-culturing to determine the procedures most suitable to culture conditions.

Instructions for Use

  1. Pre-warm Cellstripper® to incubator temperature. This can be accomplished using a 37°C water bath or by simply placing the bottle in the incubator for a short time.
  2. Remove the spent medium from the culture vessel (flask, petri dish, etc.).
  3. Add a small amount of dissociation solution to the vessel without disturbing the monolayer. This is usually best performed by slowly adding the solution to the side of the flask opposite the monolayer or to the side of a petri plate. Gently swirl the vessel to cover the monolayer with the solution. Discard the solution from the vessel.
  4. Repeat Step #3. Leave a small amount of Cellstripper® solution in the vessel, enough just to cover the monolayer.
  5. Incubate the cells at 37°C, observing microscopically, until the cells begin to round up. Tapping the side of the vessel may facilitate removal of difficult cell lines.
  6. After detachment, disperse the cells into suspension by pipetting repeatedly. Continue with the normal procedure.
Cat.No. Name Comments Pkg Size PK   Seal Color
23-25-056-CI Cell Stripper, liquid Nonenzymatic Cell Dissociation Solution 6 bottles/100mL $97.36
Add To Favorites

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price

Request Literature

Please fill out the form below if you want to request literature from Krackeler Scientific.


Quick Order

* Required

New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top