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Minimum Essential Medium Alpha Medium (AMEM)

By Corning® cellgro®

Shipped at 15 to 30°C; store at 2 to 8°C.

Cat.No. Description Pack Size Shipped Storage Intended Use
Minimum Essential Medium (MEM) Alpha Medium, 1X
23-10-022-CV with Earle's salts, ribonucleosides, deoxyribonucleosides, & L-glutamine 6 x 500 mL A R I
23-15-012-CV with Earle's salts
without ribonucleosides, deoxyribonucleosides, & L-glutamine
6 x 500 mL A R I
Minimum Essential Medium (MEM) Alpha Medium, Powder
23-50-012-PB with Earle's salts & L-glutamine
without ribonucleosides, deoxyribonucleosides, & sodium bicarbonate
1 x 10 L A R IVD
23-50-012-PC with Earle's salts & L-glutamine
without ribonucleosides, deoxyribonucleosides, & sodium bicarbonate
1 x 50 L A R IVD
23-90-012-PB with Earle's salts
without ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, & sodium bicarbonate
1 x 10 L A R IVD
23-90-012-PC with Earle's salts
without ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, & sodium bicarbonate
1 x 50 L A R IVD

F = -5 to -20°C
R = 2 to 8°C
A = 15 to 30°C
* = Glass
** = Flexible Media Bag
I = Sterile, IVD Product
NI = Sterile, Research Use Only, Not for IVD Use
IVD = In Vitro Diagnostic
Res = Research Use Only, Not for IVD Use

Minimum Essential Medium, Alpha Modification Formulation

Catalog No. 23-10-022
1X, Liquid
AMEM
23-15-012
1X, Liquid
AMEM
23-50-012
Powder
AMEM
23-90-012
Powder
AMEM
Inorganic Salts
CaCl2 (anhydrous) 200.00mg/L 200.00mg/L 200.00mg/L 200.00mg/L
KCl 400.00 400.00 400.00 400.00
MgSO4 (anhydrous) 97.70 97.70 97.70 97.70
NaCl 6800.00 6800.00 6800.00 6800.00
NaH2PO4•H2O 140.00 140.00 140.00 140.00
NaHCO3 2200.00 2200.00 - -
Amino acids
L-Alanine 25.00 25.00 25.00 25.00
L-Arginine•HCl 126.40 126.40 126.40 126.40
L-Asparagine•H2O 50.00 50.00 50.00 50.00
L-Aspartic acid 30.00 30.00 30.00 30.00
L-Cysteine•HCl•H2O 100.00 100.00 100.00 100.00
L-Cystine•2HCl 31.20 31.20 31.20 31.20
L-Glutamic acid 75.00 75.00 75.00 75.00
L-Glutamine 292.00 - 292.00 -
Glycine 50.00 50.00 50.00 50.00
L-Histidine•HCl•H2O 41.90 41.90 41.90 41.90
L-Isoleucine 52.50 52.50 52.50 52.50
L-Leucine 52.50 52.50 52.50 52.50
L-Lysine•HCl 72.50 72.50 72.50 72.50
L-Methionine 15.00 15.00 15.00 15.00
L-Phenylalanine 32.50 32.50 32.50 32.50
L-Proline 40.00 40.00 40.00 40.00
L-Serine 25.00 25.00 25.00 25.00
L-Threonine 47.60 47.60 47.60 47.60
L-Tryptophan 10.00 10.00 10.00 10.00
L-Tyrosine•2Na•2H2O 51.90 51.90 51.90 51.90
L-Valine 46.80 46.80 46.80 46.80
Vitamins
Ascorbic acid 50.00 50.00 50.00 50.00
Biotin 0.10 0.10 0.10 0.10
D-Calcium pantothenate 1.00 1.00 1.00 1.00
Choline chloride 1.00 1.00 1.00 1.00
Folic acid 1.00 1.00 1.00 1.00
i-Inositol 2.00 2.00 2.00 2.00
Nicotinamide 1.00 1.00 1.00 1.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00
Riboflavin 0.10 0.10 0.10 0.10
Thiamine•HCl 1.00 1.00 1.00 1.00
Vitamin B12 1.36 1.36 1.36 1.36
Other
D-Glucose 1000.00 1000.00 1000.00 1000.00
Lipoic acid 0.20 0.20 0.20 0.20
Phenol red, Na 10.00 10.00 10.00 -
Sodium pyruvate 110.00 110.00 110.00 110.00
Nucleosides
Thymidine 10.00 - - -
Adenosine 10.00 - - -
Cytidine 10.00 - - -
Guanosine 10.00 - - -
Uridine (anhydrous) 10.00 - - -
2'-Deoxyadenosine 10.00 - - -
2'-Deoxycytidine•HCl 11.00 - - -
2'-Deoxyguanosine 10.00 - - -
Add
NaHCO3
Powder (g/L)
7.5% Solution (mL/L)

-
-

-
-

2.20
29.33

2.20
29.33
L-Glutamine
Powder (mg/L)
200 mM Solution (mL/L)

-
-

292.00
10.00

-
-

292.00
10.00
Specifications
pH (before buffer) N/A N/A 3.5±0.5 3.5±0.5
pH (after buffer) 7.2±0.2 7.2±0.2 7.0±0.5 7.0±0.5
Osmolality (mOsm) 290±15 290±30 290±30 290±30
Grams of powder required to prepare 1L (1X Solution) N/A N/A 10.09 9.78
                 
Cat.No. Name Pkg Size EA     PK    
23-10-022-CV AMEM w/ Earle's salts, ribonucleosides, deoxyribonucleosides, L-glutamine 6-pack/500mL bottles       $166.13
1/PK
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23-15-012-CV AMEM w/ Earle's salts, w/o ribonucleosides, deoxyribonucleosides, L-glutamine 6-pack/500mL bottles       $131.84
1/PK
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23-50-012-PB AMEM w/ Earle's salts, L-glutamine, w/o ribonucleosides, deoxyribonucleosides, sodium bicarbonate 10L $27.83
1/EA
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23-50-012-PC AMEM w/ Earle's salts, L-glutamine, w/o ribonucleosides, deoxyribonucleosides, sodium bicarbonate 50L $59.04
1/EA
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23-90-012-PB AMEM w/ Earle's salts, w/o ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, sodium bicarbonate 10L $26.57
1/EA
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23-90-012-PC AMEM w/ Earle's salts, w/o ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, sodium bicarbonate 50L $182.87
1/EA
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