Corning® Minimum Essential Medium (MEM)

Medium is shipped at 15 to 30°C; store at 2 to 8°C.

Minimum Essential Medium Formulation

Catalog No.	23-10-010 1X, Liquid EMEM	23-15-010 1X, Liquid EMEM	23-15-015 1X, Liquid SMEM	23-17-303 1X, Liquid EMEM	23-17-305 1X, Liquid EMEM
Inorganic Salts
CaCl2 (anhydrous)	200.00mg/L	200.00mg/L	-	200.00mg/L	200.00mg/L
KCl	400.00	400.00	400.00mg/L	400.00	400.00
MgSO4 (anhydrous)	97.70	97.70	-	97.70	97.70
NaCl	6800.00	6800.00	6800.00	6800.00	6800.00
NaH2PO4•H2O	140.00	140.00	1400.00	140.00	140.00
Na2HPO4	-	-	-	-	-
NaHCO3	2200.00	2200.00	2200.00	2200.00	2200.00
Amino acids
L-Arginine•HCl	126.40mg/L	126.40mg/L	126.40mg/L	126.40mg/L	126.40mg/L
L-Cystine•2HCl	31.20	31.20	31.20	31.20	31.20
L-Glutamine	292.00	-	-	-	-
L-Histidine•HCl•H2O	41.90	41.90	41.90	41.90	41.90
L-Isoleucine	52.50	52.50	52.50	52.50	52.50
L-Leucine	52.50	52.50	52.50	52.50	52.50
L-Lysine•HCl	72.50	72.50	72.50	72.50	72.50
L-Methionine	15.00	15.00	15.00	-	15.00
L-Phenylalanine	32.50	32.50	32.50	32.50	32.50
L-Threonine	47.60	47.60	47.60	47.60	47.60
L-Tryptophan	10.00	10.00	10.00	10.00	10.00
L-Tyrosine•2Na•2H2O	51.90	51.90	51.90	51.90	51.90
L-Valine	46.80	46.80	46.80	46.80	46.80
Vitamins
D-Calcium pantothenate	1.00mg/L	1.00mg/L	1.00mg/L	1.00mg/L	1.00mg/L
Choline chloride	1.00	1.00	1.00	1.00	1.00
Folic acid	1.00	1.00	1.00	1.00	1.00
i-Inositol	2.00	2.00	2.00	2.00	2.00
Nicotinamide	1.00	1.00	1.00	1.00	1.00
Pyridoxine•HCl	1.00	1.00	1.00	1.00	1.00
Riboflavin	0.10	0.10	0.10	0.10	0.10
Thiamine•HCl	1.00	1.00	1.00	1.00	1.00
Other
D-Glucose	1000.00mg/L	1000.00mg/L	1000.00mg/L	1000.00mg/L	1000.00mg/L
Phenol red, Na	10.00	10.00	10.00	10.00	-
Add
NaHCO3 Powder (g/L) 7.5% Solution (mL/L)	- -	- -	- -	- -	- -
L-Glutamine Powder 200 mM Solution (mL/L)	- -	292.00mg/L 10.00mL/L	292.00mg/L 10.00mL/L	292.00mg/L 10.00mL/L	292.00mg/L 10.00mL/L
Specifications
pH (before buffer)	N/A	N/A	N/A	N/A	N/A
pH (after buffer)	7.2±0.2	7.2±0.2	7.2±0.2	7.2±0.2	7.2±0.2
Osmolality (mOsm)	290±30	290±30	290±30	290±30	290±30
Grams of powder required to prepare 1L (1X Solution)	N/A	N/A	N/A	N/A	N/A

Minimum Essential Medium Formulation

Catalog No.	23-50-004 Powder GMEM	23-50-010 Powder EMEM	23-50-011 Powder EMEM	23-50-019 Powder HMEM	23-51-010 Powder Autoclavable EMEM	23-90-009 Powder EMEM
Inorganic Salts
CaCl2 (anhydrous)	200.00mg/L	200.00mg/L	200.00mg/L	140.00mg/L	200.00mg/L	200.00mg/L
Fe(NO3)3•9H2O	0.10	-	-	-	-	-
KCl	400.00	400.00	400.00	400.00	400.00	400.00
KH2PO4	-	-	-	60.00	-	-
MgSO4 (anhydrous)	97.67	97.70	97.70	97.67	97.00	97.70
NaCl	6400.00	6800.00	6800.00	8000.00	6800.00	6800.00
NaH2PO4•H2O	124.00	140.00	140.00	-	140.00	140.00
Na2HPO4 (anhydrous)	-	-	-	47.88	-	-
NaHCO3	-	-	-	-	-	-
Na succinate•6H2O	-	-	-	-	100.00	-
Amino acids
L-Alanine	-	-	8.90mg/L	-	-	-
L-Arginine•HCl	42.00mg/L	126.40mg/L	126.40	126.00mg/L	126.40mg/L	126.40mg/L
L-Asparagine•H2O	-	-	15.00	-	-	-
L-Aspartic acid	-	-	13.30	-	-	-
L-Cystine•2HCl	31.28	31.20	31.20	31.29	31.10	31.20
L-Glutamic acid	-	-	14.70	-	-	-
L-Glutamine	292.00	292.00	292.00	292.00	-	-
Glycine	-	-	7.50	-	-	-
L-Histidine•HCl•H2O	21.00	41.90	41.90	42.00	41.90	41.90
L-Isoleucine	52.40	52.50	52.50	52.00	52.50	52.50
L-Leucine	52.40	52.50	52.50	52.00	52.50	52.50
L-Lysine•HCl	73.10	72.50	72.50	72.50	73.06	72.50
L-Methionine	15.00	15.00	15.00	15.00	14.90	15.00
L-Phenylalanine	33.00	32.50	32.50	32.00	33.02	32.50
L-Proline	-	-	11.50	-	-	-
L-Serine	-	-	10.50	-	-	-
L-Threonine	47.60	47.60	47.60	48.00	47.64	47.60
L-Tryptophan	8.00	10.00	10.00	10.00	10.20	10.00
L-Tyrosine•2Na•2H2O	52.19	51.90	51.90	51.90	-	51.90
L-Tyrosine, free base	-	-	-	-	36.00	-
L-Valine	46.80	46.80	46.80	46.00	46.90	46.80
Vitamins
Choline bitartrate	-	-	-	-	1.80mg/L	-
D-Calcium pantothenate	2.00mg/L	1.00mg/L	1.00mg/L	1.00mg/L	1.00	1.00mg/L
Choline chloride	2.00	1.00	1.00	1.00	-	1.00
Folic acid	2.00	1.00	1.00	1.00	1.00	1.00
i-Inositol	3.60	2.00	2.00	2.00	2.00	2.00
Nicotinamide	2.00	1.00	1.00	1.00	1.00	1.00
Pyridoxine•HCl	2.00	1.00	1.00	1.00	1.00	1.00
Riboflavin	0.20	0.10	0.10	0.10	0.10	0.10
Thiamine•HCl	2.00	1.00	1.00	1.00	1.00	1.00
Other
D-Glucose	4500.00mg/L	1000.00mg/L	1000.00mg/L	1000.00mg/L	1000.00mg/L	1000.00mg/L
Phenol red, Na	15.00	10.00	10.00	10.00	6.00	-
Sodium pyruvate	-	-	-	-	-	-
Succinic acid	-	-	-	-	75.00	-
Add
NaHCO3 Powder 7.5% Solution	2.75g/L 36.67mL/L	2.20g/L 29.33mL/L	2.20g/L 29.33mL/L	2.20g/L 29.33mL/L	2.20g/L 29.33mL/L	2.20g/L 29.33mL/L
L-Glutamine Powder 200 mM Solution	- -	- -	- -	- -	292.00mg/L 10.00mL/L	292.00mg/L 10.00mL/L
Specifications
pH (before buffer)	6.0±0.5	6.0±0.5	5.5±0.5	6.7±0.2	INFO	6.0±0.5
pH (after buffer)	7.5±0.5	7.5±0.5	7.5±0.5	7.2±0.2	INFO	7.5±0.5
Osmolality (mOsm)	290±30	290±30	290±30	285±15	INFO	290±30
Grams of powder required to prepare 1L (1X Solution)	12.52	9.53	9.61	10.63	9.39	9.23

Add to Cart
Cat.No.	Name	Comments	Pkg Size	EA			PK			CS
23-10-009-CV	Minimum Essential Medium, 500 mL,									$129.30 0/CS		Add To Favorites
23-10-010-CM	MEM with Earle's Salts and L-glutamine		6-pack/1L bottles				$198.22 1/PK		Add To Favorites
23-10-010-CV	MEM with Earle's Salts and L-glutamine		6-pack/500mL bottles				$127.32 1/PK		Add To Favorites
23-15-010-CM	MEM with Earle's Salts w/o L-glutamine		6-pack/1L bottles				$231.80 1/PK		Add To Favorites
23-15-010-CV	MEM with Earle's Salts w/o L-glutamine		6-pack/500mL bottles				$117.98 1/PK		Add To Favorites
23-15-015-CV	MEM w/o L-glutamine, calcium, magnesium	for suspension cultures	6-pack/500mL bottles				$413.07 1/PK		Add To Favorites
23-17-305-CV	MEM w/ Earle's salts, w/o L-glutamine, phenol red		6-pack/500mL bottles				$160.22 1/PK		Add To Favorites
23-50-010-PB	MEM w/ Earle's salts, L-glutamine, w/o sodium bicarbonate	powder	10L bottle	$43.23 1/EA		Add To Favorites
23-50-010-PC	MEM w/ Earle's salts, L-glutamine, w/o sodium bicarbonate	powder	50L bottle	$143.30 1/EA		Add To Favorites
23-50-011-PB	MEM w/ Earle's salts, L-glutamine, nonessential amino acids, w/o sodium bicarbonate	powder	10L bottle	$41.59 1/EA		Add To Favorites
23-50-011-PC	MEM w/ Earle's salts, L-glutamine, nonessential amino acids, w/o sodium bicarbonate	powder	50L bottle	$63.52 1/EA		Add To Favorites
23-51-010-PC	MEM, w/ Earle's salts, w/o L-glutamine, sodium bicarbonate	powder (autoclavable)	50L bottle	$64.73 1/EA		Add To Favorites
23-90-009-PB	MEM w/ Earle's salts, w/o L-glutamine, phenol red, sodium bicarbonate	powder	10L bottle	$48.04 1/EA		Add To Favorites
23-90-009-PC	MEM w/ Earle's salts, w/o L-glutamine, phenol red, sodium bicarbonate	powder	50L bottle	$0.00 1/EA CALL FOR PRICE		Add To Favorites